UniProt Accession

 NARY_ECOLI; P19318; P78267 

Genbank Protein ID

 X17110; U00096; AP009048 

Genbank Nucleotide ID

 CAA34965.1; AAC74549.1; BAA15104.1 

Protein Name

 Respiratory nitrate reductase 2 beta chain 

Protein Synonyms/Alias

  

Gene Name

 narY 

Gene Synonyms/Alias

 b1467; JW1462 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
337	YKMAMDWKLALPLHP	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit. 

Sequence Annotation

 DOMAIN 7 35 4Fe-4S ferredoxin-type 1.
 DOMAIN 174 205 4Fe-4S ferredoxin-type 2.
 DOMAIN 207 236 4Fe-4S ferredoxin-type 3.
 METAL 16 16 Iron-sulfur 1 (4Fe-4S) (By similarity).
 METAL 19 19 Iron-sulfur 1 (4Fe-4S) (By similarity).
 METAL 22 22 Iron-sulfur 1 (4Fe-4S) (By similarity).
 METAL 26 26 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 183 183 Iron-sulfur 3 (4Fe-4S) (By similarity).
 METAL 186 186 Iron-sulfur 3 (4Fe-4S) (By similarity).
 METAL 191 191 Iron-sulfur 3 (4Fe-4S) (By similarity).
 METAL 195 195 Iron-sulfur 4 (3Fe-4S) (By similarity).
 METAL 216 216 Iron-sulfur 4 (3Fe-4S) (By similarity).
 METAL 222 222 Iron-sulfur 4 (3Fe-4S) (By similarity).
 METAL 226 226 Iron-sulfur 3 (4Fe-4S) (By similarity).
 METAL 243 243 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 246 246 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 258 258 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 262 262 Iron-sulfur 1 (4Fe-4S) (By similarity).  

Keyword

 3Fe-4S; 4Fe-4S; Cell membrane; Complete proteome; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Nitrate assimilation; Oxidoreductase; Reference proteome; Repeat; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 514 AA 

Protein Sequence

Gene Ontology

 GO:0009325; C:nitrate reductase complex; IEA:InterPro.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0051538; F:3 iron, 4 sulfur cluster binding; IPI:EcoCyc.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IPI:EcoCyc.
 GO:0009055; F:electron carrier activity; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008940; F:nitrate reductase activity; IEA:EC.
 GO:0009061; P:anaerobic respiration; IDA:EcoCyc.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
 GO:0006974; P:response to DNA damage stimulus; IEP:EcoliWiki. 

Interpro

 IPR017896; 4Fe4S_Fe-S-bd.
 IPR006547; NO3_Rdtase_bsu. 

Pfam

 PF13247; Fer4_11 

SMART

  

PROSITE

 PS00198; 4FE4S_FER_1
 PS51379; 4FE4S_FER_2 

PRINTS