CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022096
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vacuolar protein sorting-associated protein 45 
Protein Synonyms/Alias
 h-VPS45; hlVps45 
Gene Name
 VPS45 
Gene Synonyms/Alias
 VPS45A; VPS45B 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
64SQNREIMKHLKAICFubiquitination[1]
76ICFLRPTKENVDYIIubiquitination[1]
170TALLLSLKKCPMIRYubiquitination[1, 2, 3, 4]
171ALLLSLKKCPMIRYQubiquitination[1]
185QLSSEAAKRLAECVKubiquitination[1, 3]
192KRLAECVKQVITKEYubiquitination[1]
197CVKQVITKEYELFEFubiquitination[1]
257SRVPGISKDLREVVLubiquitination[1, 2, 3, 4, 5]
296NLMEDFQKKKPKEQQubiquitination[1, 3]
297LMEDFQKKKPKEQQKubiquitination[1]
304KKPKEQQKLESIADMubiquitination[1]
312LESIADMKAFVENYPubiquitination[1, 3]
322VENYPQFKKMSGTVSubiquitination[1, 6]
323ENYPQFKKMSGTVSKubiquitination[1]
371SSALQNIKRLLQNPKubiquitination[1, 7]
378KRLLQNPKVTEFDAAubiquitination[1, 3, 6]
432AVVEYGGKRVRGSDLubiquitination[1]
443GSDLFSPKDAVAITKubiquitination[1, 3]
450KDAVAITKQFLKGLKubiquitination[1, 2, 3, 4, 6, 8]
483HLIKGRLKENLYPYLubiquitination[1, 2, 4, 6, 7, 8]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
 May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. 
Sequence Annotation
  
Keyword
 Complete proteome; Endosome; Golgi apparatus; Membrane; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 570 AA 
Protein Sequence
MNVVFAVKQY ISKMIEDSGP GMKVLLMDKE TTGIVSMVYT QSEILQKEVY LFERIDSQNR 60
EIMKHLKAIC FLRPTKENVD YIIQELRRPK YTIYFIYFSN VISKSDVKSL AEADEQEVVA 120
EVQEFYGDYI AVNPHLFSLN ILGCCQGRNW DPAQLSRTTQ GLTALLLSLK KCPMIRYQLS 180
SEAAKRLAEC VKQVITKEYE LFEFRRTEVP PLLLILDRCD DAITPLLNQW TYQAMVHELL 240
GINNNRIDLS RVPGISKDLR EVVLSAENDE FYANNMYLNF AEIGSNIKNL MEDFQKKKPK 300
EQQKLESIAD MKAFVENYPQ FKKMSGTVSK HVTVVGELSR LVSERNLLEV SEVEQELACQ 360
NDHSSALQNI KRLLQNPKVT EFDAARLVML YALHYERHSS NSLPGLMMDL RNKGVSEKYR 420
KLVSAVVEYG GKRVRGSDLF SPKDAVAITK QFLKGLKGVE NVYTQHQPFL HETLDHLIKG 480
RLKENLYPYL GPSTLRDRPQ DIIVFVIGGA TYEEALTVYN LNRTTPGVRI VLGGTTVHNT 540
KSFLEEVLAS GLHSRSKESS QVTSRSASRR 570 
Gene Ontology
 GO:0010008; C:endosome membrane; TAS:Reactome.
 GO:0005794; C:Golgi apparatus; ISS:BHF-UCL.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; ISS:BHF-UCL.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0006886; P:intracellular protein transport; NAS:UniProtKB.
 GO:0006904; P:vesicle docking involved in exocytosis; IEA:InterPro. 
Interpro
 IPR027482; Sec-1-like_dom2.
 IPR001619; Sec1-like. 
Pfam
 PF00995; Sec1 
SMART
  
PROSITE
  
PRINTS