UniProt Accession

 SYNC_MOUSE; Q8BP47; Q3T9A7; Q7TPX0; Q9CRY5 

Genbank Protein ID

 AK013880; AK077699; AK172657; BC052849 

Genbank Nucleotide ID

 BAB29032.1; BAC36965.1; BAE43117.1; AAH52849.1 

Protein Name

 Asparagine--tRNA ligase, cytoplasmic 

Protein Synonyms/Alias

 Asparaginyl-tRNA synthetase; AsnRS 

Gene Name

 Nars 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
49	KALMTVGKEPFPTIY	ubiquitination	[1]
71	ERWDVISKSQMKNIK	acetylation	[2]
71	ERWDVISKSQMKNIK	succinylation	[2]
115	EAKKIIIKNDPSLPE	acetylation	[3]
115	EAKKIIIKNDPSLPE	ubiquitination	[1]
140	GYRGQRVKVFGWVHR	acetylation	[3]
255	IRGENMSKILKARSM	acetylation	[3]
255	IRGENMSKILKARSM	ubiquitination	[1]
393	YELNPNFKPPKRPFR	ubiquitination	[1]
456	CRFPVEIKSFYMQRC	ubiquitination	[1]
501	EEILEGYKREGIDPA	acetylation	[2]
501	EEILEGYKREGIDPA	ubiquitination	[1]
518	YWYTDQRKYGTCPHG	acetylation	[3]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

  

Sequence Annotation

 MOD_RES 72 72 Phosphoserine (By similarity).
 MOD_RES 255 255 N6-acetyllysine (By similarity).
 MOD_RES 493 493 Phosphoserine.  

Keyword

 Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 559 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004816; F:asparagine-tRNA ligase activity; IEA:EC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0006421; P:asparaginyl-tRNA aminoacylation; IEA:InterPro. 

Interpro

 IPR004364; aa-tRNA-synt_II.
 IPR018150; aa-tRNA-synt_II-like.
 IPR006195; aa-tRNA-synth_II.
 IPR004522; Asn-tRNA-ligase_IIb.
 IPR002312; Asp/Asn-tRNA-synth_IIb.
 IPR012340; NA-bd_OB-fold.
 IPR004365; NA-bd_OB_tRNA-helicase. 

Pfam

 PF00152; tRNA-synt_2
 PF01336; tRNA_anti 

SMART

  

PROSITE

 PS50862; AA_TRNA_LIGASE_II 

PRINTS

 PR01042; TRNASYNTHASP.