CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023959
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA repair and recombination protein RAD54B 
Protein Synonyms/Alias
 RAD54 homolog B 
Gene Name
 RAD54B 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
157KGKSFILKNLEGKDIubiquitination[1]
226HSSQVARKCFSNPFKubiquitination[1]
252QNDFQNCKPRHDPYTubiquitination[1]
490GSLSSYRKIYEEPIIubiquitination[2]
590ICIGALKKLCNHPCLubiquitination[1]
603CLLFNSIKEKECSSTubiquitination[1]
621NEEKSLYKGLLSVFPubiquitination[1]
639NPLLFTEKESGKLQVubiquitination[2]
643FTEKESGKLQVLSKLubiquitination[1]
777TTGTIEEKIYQRQISubiquitination[1]
864HQKSNSLKPLSMSQLubiquitination[1]
872PLSMSQLKQWKHFSGubiquitination[1]
875MSQLKQWKHFSGDHLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Involved in DNA repair and mitotic recombination. May play an active role in recombination processes in concert with other members of the RAD52 epistasis group. 
Sequence Annotation
 DOMAIN 313 480 Helicase ATP-binding.
 DOMAIN 649 810 Helicase C-terminal.
 NP_BIND 326 333 ATP (Potential).
 MOTIF 431 434 DEGH box.  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 910 AA 
Protein Sequence
MRRSAAPSQL QGNSFKKPKF IPPGRSNPGL NEEITKLNPD IKLFEGVAIN NTFLPSQNDL 60
RICSLNLPSE ESTREINNRD NCSGKYCFEA PTLATLDPPH TVHSAPKEVA VSKEQEEKSD 120
SLVKYFSVVW CKPSKKKHKK WEGDAVLIVK GKSFILKNLE GKDIGRGIGY KFKELEKIEE 180
GQTLMICGKE IEVMGVISPD DFSSGRCFQL GGGSTAISHS SQVARKCFSN PFKSVCKPSS 240
KENRQNDFQN CKPRHDPYTP NSLVMPRPDK NHQWVFNKNC FPLVDVVIDP YLVYHLRPHQ 300
KEGIIFLYEC VMGMRMNGRC GAILADEMGL GKTLQCISLI WTLQCQGPYG GKPVIKKTLI 360
VTPGSLVNNW KKEFQKWLGS ERIKIFTVDQ DHKVEEFIKS IFYSVLIISY EMLLRSLDQI 420
KNIKFDLLIC DEGHRLKNSA IKTTTALISL SCEKRIILTG TPIQNDLQEF FALIDFVNPG 480
ILGSLSSYRK IYEEPIILSR EPSASEEEKE LGERRAAELT CLTGLFILRR TQEIINKYLP 540
PKIENVVFCR PGALQIELYR KLLNSQVVRF CLQGLLENSP HLICIGALKK LCNHPCLLFN 600
SIKEKECSST CDKNEEKSLY KGLLSVFPAD YNPLLFTEKE SGKLQVLSKL LAVIHELRPT 660
EKVVLVSNYT QTLNILQEVC KRHGYAYTRL DGQTPISQRQ QIVDGFNSQH SSFFIFLLSS 720
KAGGVGLNLI GGSHLILYDI DWNPATDIQA MSRVWRDGQK YPVHIYRLLT TGTIEEKIYQ 780
RQISKQGLCG AVVDLTKTSE HIQFSVEELK NLFTLHESSD CVTHDLLDCE CTGEEVHTGD 840
SLEKFIVSRD CQLGPHHQKS NSLKPLSMSQ LKQWKHFSGD HLNLTDPFLE RITENVSFIF 900
QNITTQATGT 910 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003678; F:DNA helicase activity; TAS:ProtInc.
 GO:0015616; F:DNA translocase activity; IDA:MGI.
 GO:0003724; F:RNA helicase activity; TAS:ProtInc.
 GO:0000724; P:double-strand break repair via homologous recombination; IDA:MGI.
 GO:0006312; P:mitotic recombination; TAS:ProtInc.
 GO:0007131; P:reciprocal meiotic recombination; TAS:ProtInc. 
Interpro
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000330; SNF2_N. 
Pfam
 PF00271; Helicase_C
 PF00176; SNF2_N 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS