UniProt Accession

 CYPH_YEAST; P14832; D6VSD6 

Genbank Protein ID

 X17505; M30513; Z50046; AY557665; BK006938 

Genbank Nucleotide ID

 CAA35545.1; AAA34528.1; CAA90376.1; AAS55991.1; DAA11996.1 

Protein Name

 Peptidyl-prolyl cis-trans isomerase 

Protein Synonyms/Alias

 PPIase; Cyclophilin; CPH; Cyclosporin A-binding protein; PPI-II; Rotamase 

Gene Name

 CPR1 

Gene Synonyms/Alias

 CPH1; CYP1; SCC1; YDR155C; YD8358.10C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
21	PIGRVVFKLYNDIVP	acetylation	[1]
21	PIGRVVFKLYNDIVP	ubiquitination	[2]
29	LYNDIVPKTAENFRA	acetylation	[1]
29	LYNDIVPKTAENFRA	ubiquitination	[2, 3, 4]
42	RALCTGEKGFGYAGS	acetylation	[1]
42	RALCTGEKGFGYAGS	ubiquitination	[2, 4]
74	AGNGTGGKSIYGGKF	acetylation	[1]
74	AGNGTGGKSIYGGKF	ubiquitination	[2]
80	GKSIYGGKFPDENFK	acetylation	[1]
80	GKSIYGGKFPDENFK	ubiquitination	[2, 4]
87	KFPDENFKKHHDRPG	acetylation	[1]
87	KFPDENFKKHHDRPG	ubiquitination	[2]
123	PCPWLDGKHVVFGEV	ubiquitination	[4]
138	VDGYDIVKKVESLGS	acetylation	[1]
138	VDGYDIVKKVESLGS	ubiquitination	[4]
139	DGYDIVKKVESLGSP	acetylation	[1]
139	DGYDIVKKVESLGSP	ubiquitination	[2, 4]
151	GSPSGATKARIVVAK	acetylation	[1]
151	GSPSGATKARIVVAK	ubiquitination	[2, 4]
158	KARIVVAKSGEL***	ubiquitination	[2, 4, 5]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis.
 Ziv I, Matiuhin Y, Kirkpatrick DS, Erpapazoglou Z, Leon S, Pantazopoulou M, Kim W, Gygi SP, Haguenauer-Tsapis R, Reis N, Glickman MH, Kleifeld O.
 Mol Cell Proteomics. 2011 May;10(5):M111.009753. [PMID: 21427232]
 [4] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [5] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Involved in histone deacetylase complexes, suggesting a function in chromatin. Imports fructose-1,6- bisphosphatase (FBPase) into the intermediate vacuole import and degradation (Vid) vesicles. 

Sequence Annotation

 DOMAIN 5 161 PPIase cyclophilin-type.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 71 71 Phosphothreonine.
 MOD_RES 142 142 Phosphoserine.
 MOD_RES 145 145 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cyclosporin; Cytoplasm; Direct protein sequencing; Isomerase; Phosphoprotein; Protein transport; Reference proteome; Rotamase; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 162 AA 

Protein Sequence

Gene Ontology

 GO:0005758; C:mitochondrial intermembrane space; IDA:SGD.
 GO:0034967; C:Set3 complex; IDA:SGD.
 GO:0016018; F:cyclosporin A binding; IMP:SGD.
 GO:0003755; F:peptidyl-prolyl cis-trans isomerase activity; IDA:SGD.
 GO:0030437; P:ascospore formation; IMP:SGD.
 GO:0016575; P:histone deacetylation; IDA:SGD.
 GO:0045836; P:positive regulation of meiosis; IMP:SGD.
 GO:0006457; P:protein folding; IEA:UniProtKB-KW.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 

Interpro

 IPR002130; Cyclophilin-like_PPIase_dom.
 IPR024936; Cyclophilin-type_PPIase.
 IPR020892; Cyclophilin-type_PPIase_CS. 

Pfam

 PF00160; Pro_isomerase 

SMART

  

PROSITE

 PS00170; CSA_PPIASE_1
 PS50072; CSA_PPIASE_2 

PRINTS

 PR00153; CSAPPISMRASE.