CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000903
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleolar pre-ribosomal-associated protein 1 
Protein Synonyms/Alias
 Nucleolar protein 254 kDa; URB1 ribosome biogenesis 1 homolog 
Gene Name
 URB1 
Gene Synonyms/Alias
 C21orf108; KIAA0539; NOP254; NPA1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MGVPKRKASGGQDGubiquitination[1]
23ASSAGAAKRARKEELubiquitination[2]
40VRFKAQLKDPQGPGPubiquitination[1, 2]
57EAFVSAAKKLPREDVubiquitination[1]
58AFVSAAKKLPREDVYubiquitination[2, 3]
177SHFDLNKKTLYTLVTubiquitination[2]
185TLYTLVTKRDSKGVYubiquitination[1, 2, 4]
189LVTKRDSKGVYDVRQubiquitination[1, 2]
248NILLSTLKTKVVHNKubiquitination[1]
250LLSTLKTKVVHNKNIubiquitination[2]
300VSAEEAGKTMVRELVubiquitination[1, 2, 5]
376DLLNKYFKEVTFSFIubiquitination[6, 7]
387FSFIPRAKSTWLNNIubiquitination[2, 5]
395STWLNNIKLLNKIYEubiquitination[1, 2]
399NNIKLLNKIYEAQPEubiquitination[1, 2, 3, 4, 5, 8]
585FDFSKLLKGVISEQGubiquitination[1, 4, 5]
616ALELPASKFLWLKAQubiquitination[1, 2, 3, 4, 5, 8]
621ASKFLWLKAQEGPDAubiquitination[1, 4]
1085AITQSVLKELQNRRAubiquitination[1, 2, 3, 4, 6, 7]
1100GPATSPPKTPPQLEAubiquitination[2, 4, 5]
1142LVTQQPTKSPGKERHubiquitination[1, 2, 4, 5]
1336LVPFARAKDLSVLMDubiquitination[1, 2, 5]
1356LHTPSSHKRWIVADSubiquitination[2]
1409DNTQNQEKEMLLRLNubiquitination[1]
1432VDPGDWQKFVKKGLKubiquitination[1, 2, 3, 4, 5]
1436DWQKFVKKGLKFRYQubiquitination[2]
1555LRAYEQNKLSLINFRubiquitination[1, 2, 4]
1575PAAVEHHKTCRSLGRubiquitination[2, 4, 5]
1627DTQELIFKDKSRVDLubiquitination[1, 2, 3, 5]
1629QELIFKDKSRVDLDGubiquitination[2]
1766HEYLNMDKVPGFYQFubiquitination[3]
1782YSSDFEQKTEQKWVFubiquitination[1, 3]
1786FEQKTEQKWVFGVLRubiquitination[6, 7]
1799LRQGIRDKQCYELCAubiquitination[2, 4, 5]
1909PSSQEPAKRLALHLVubiquitination[2, 4, 5]
1976NETVLSTKDVLVLLHubiquitination[1, 4]
1993SLIERDLKLQEDLRAubiquitination[1, 2, 5]
2004DLRAAIEKAQARELMubiquitination[5]
2012AQARELMKMLKDKNKubiquitination[2]
2019KMLKDKNKPVMPARAubiquitination[2]
2056ASTLETCKGLLRSILubiquitination[2]
2141VVVADLLKDSAVRSSubiquitination[1, 2, 4, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
 MOD_RES 1143 1143 Phosphoserine.  
Keyword
 Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2271 AA 
Protein Sequence
MGVPKRKASG GQDGAASSAG AAKRARKEEL TGVRFKAQLK DPQGPGPGLE AFVSAAKKLP 60
REDVYDVVEG YIKISVECVE IFQLLSGEKR PESETMLIFQ VFEAILLRTA SDLSHFHVVG 120
TNIVKKLMNN HMKLICESLY ASGYRLARAC LSLMTAMVTQ GPEAARDVCS HFDLNKKTLY 180
TLVTKRDSKG VYDVRQAYVQ FALSFLIAGD DSTIVQVLEV KEFIPCIFSS GIKEDRISTI 240
NILLSTLKTK VVHNKNITKT QKVRFFTGQL LNHIASLYNW NGITDVNPEN VKVSAEEAGK 300
TMVRELVHNF LMDLCCSLKH GINFYDASLG TFGRGGNLTL LHFLLGLKTA ADDDLVADLV 360
VNILKVCPDL LNKYFKEVTF SFIPRAKSTW LNNIKLLNKI YEAQPEISRA FQTREFIPLP 420
RLLAMVMVTT VPLVCNKSMF TQALNLDSTS VRHTALSLIS VILKRALKTV DHCLNKEVWQ 480
ESGVYTAVMM EEFVQLFREA LSKILPDLNT VVWVWQSLKK QETKQDDKKG QKRSDGPPAA 540
CDAHQCDDAE TILLKAVLLQ VICLYQKVVP HVVMQYNFDF SKLLKGVISE QGLREEVPPI 600
LQHHMLKVAL ELPASKFLWL KAQEGPDAEI IGGERSVFYL LMKMFVTSSH LQLKSLTKLL 660
IMKILRDTGV FEHTWKELEL WLEHLENTME EDKETVIQFL ERILLTLVAN PYSYTDKASD 720
FVQEASMLQA TMTKQEADDM SIPISHIDDV LDMVDVLVEG SEGLDEEIGF TLSEDMILLT 780
FPFSAVVPAA LEARNKLLLG TGNEAAENVV TYLTAVLTDL LHTQRDPLAL CLLLQAYDKL 840
EPPCLVPCCQ QLSRFNRYYS LWIPEQAREA WLLQAQGSPS PPALPLASSF TALLQAAYES 900
QALRDEHIQV QLQATMPHLS MQQVLLAAKQ VLLYLRSTVE NFGQLGRSVG PPLLQLFLDL 960
LRRLVVHCEQ LDAQNQQRCE AARAEADLFL DMESVASLEL ANDQTLEEVL VAILRHPTLE 1020
GWFLALEQQA LPPHTLSPVL VKLLATHFSA GVLQLLAASA PILQNIGQLG LLARYSEAIT 1080
QSVLKELQNR RAGPATSPPK TPPQLEALQE LHPYMEGAQL REVTLALLSL PETHLVTQQP 1140
TKSPGKERHL NALGKTLVQL LTCSPQDQLQ SGELLWSSEY VRGLGALLPT LAVDELDTVL 1200
LHTLQRDPVL APAVGADLLD YCLARRTQAA LSIAALLLQE SCTHLLWFEQ WCLQAGPGLG 1260
LQGDLDDFLP LIHVYLQCRT RSHFTRPAGV SSAVIPVLRK TLWRQLQSRL LSTDSPPASG 1320
LYQEILAQLV PFARAKDLSV LMDRLPSLLH TPSSHKRWIV ADSISAALEG SAEELCAWRR 1380
TLLESCVKWL IVSFSGGQQD DDNTQNQEKE MLLRLNALLH ALNEVDPGDW QKFVKKGLKF 1440
RYQDHTFLKM LLTAVQLLYS PESSVRTKLI QLPVVYVMLM QHSLFLPTLL TSDGEESPDS 1500
QVKEALVDLM LTVVEMCPSV CESSHFAVLL GAYGATLSVL DQKILLLLRA YEQNKLSLIN 1560
FRVLLWGPAA VEHHKTCRSL GRSLWQQPSV GDILRLLDRD RMMQTILHFP QNRRLLPPED 1620
TQELIFKDKS RVDLDGLYDP CFLLQLFSEL TRPEFVVDCR KFLDSNALGL TVTALSSYDP 1680
QMRAIAYHVL AAYYSHLEGA RFQEQSQLLY LLDVVRNGIR TQDMRLTFTL ALFIAKAALQ 1740
ILKPEEHMYL KVSNFLLSHE YLNMDKVPGF YQFFYSSDFE QKTEQKWVFG VLRQGIRDKQ 1800
CYELCARRGI FHIILSFFHS PLCDEAAQNW ILEILQNAAQ VARSAYEIIR DYSLLTWILH 1860
ILESKFLETP LLSNVISLLH TLWVTNLGDK AVEWESQRLC QPSSQEPAKR LALHLVNEFL 1920
YVLIVLMKHL RPTLAPVQLT NFFGTLDSVL RYRATVIQAF RDMNRFTVNE TVLSTKDVLV 1980
LLHKWSLIER DLKLQEDLRA AIEKAQAREL MKMLKDKNKP VMPARAKGPR GRKRRPGEAE 2040
EMADPELMAS TLETCKGLLR SILTYWRPVI PGPDPTQEPV DSASPESDAP GPVYAAASLA 2100
VSWVLRSVAE HPLSRAEAAG LIGWLKSHIL PHPVVVADLL KDSAVRSSIF RLYSRLCGAE 2160
GLAGPVQEVA CLFNTVMLQL VAAQGRAGSP FHPAMEALSL SSLSEKDEAT QASAAFLVSL 2220
YIKDIWLGAQ RPDTLLTHVR MVCEAADDAP SSEEEAIVVL CKDAASAASD A 2271 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:UniProtKB. 
Interpro
 IPR021714; Npa1_N. 
Pfam
 PF11707; Npa1 
SMART
  
PROSITE
  
PRINTS