CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000023
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Small acidic protein 
Protein Synonyms/Alias
  
Gene Name
 SMAP 
Gene Synonyms/Alias
 C11orf58 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
49LRLMGAGKKEHTGRLubiquitination[1]
50RLMGAGKKEHTGRLVubiquitination[1]
62RLVIGDHKSTSHFRTubiquitination[2, 3, 4, 5]
74FRTGEEDKKINEELEubiquitination[4]
75RTGEEDKKINEELESubiquitination[2, 4, 5, 6]
91YQQSMDSKLSGRYRRubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
174KDAKSNYKMMFVKSSacetylation[9]
174KDAKSNYKMMFVKSSubiquitination[3, 4]
179NYKMMFVKSSGS***acetylation[9]
179NYKMMFVKSSGS***ubiquitination[4]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [9] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861
Functional Description
  
Sequence Annotation
 MOD_RES 15 15 Phosphoserine.
 MOD_RES 17 17 Phosphoserine.
 MOD_RES 127 127 Phosphoserine.
 MOD_RES 147 147 Phosphoserine.
 MOD_RES 174 174 N6-acetyllysine.
 MOD_RES 179 179 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 183 AA 
Protein Sequence
MSAARESHPH GVKRSASPDD DLGSSNWEAA DLGNEERKQK FLRLMGAGKK EHTGRLVIGD 60
HKSTSHFRTG EEDKKINEEL ESQYQQSMDS KLSGRYRRHC GLGFSEVEDH DGEGDVAGDD 120
DDDDDDSPDP ESPDDSESDS ESEKEESAEE LQAAEHPDEV EDPKNKKDAK SNYKMMFVKS 180
SGS 183 
Gene Ontology
  
Interpro
 IPR026714; SMAP. 
Pfam
  
SMART
  
PROSITE
  
PRINTS