CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002132
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Gag-Pol polyprotein 
Protein Synonyms/Alias
 Pr160Gag-Pol; Matrix protein p17; MA; Capsid protein p24; CA; Spacer peptide p2; Nucleocapsid protein p7; NC; Transframe peptide; TF; p6-pol; p6*; Protease; PR; Retropepsin; Reverse transcriptase/ribonuclease H; Exoribonuclease H; p66 RT; p51 RT; p15; Integrase; IN 
Gene Name
 gag-pol 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Human immunodeficiency virus type 1 group M subtype B (isolate HXB2 
NCBI Taxa ID
 11706 
Lysine Modification
Position
Peptide
Type
References
1405IQDNSDIKVVPRRKAacetylation[1]
1411IKVVPRRKAKIIRDYacetylation[1]
1413VVPRRKAKIIRDYGKacetylation[1]
1420KIIRDYGKQMAGDDCacetylation[1]
Reference
 [1] GCN5-dependent acetylation of HIV-1 integrase enhances viral integration.
 Terreni M, Valentini P, Liverani V, Gutierrez MI, Di Primio C, Di Fenza A, Tozzini V, Allouch A, Albanese A, Giacca M, Cereseto A.
 Retrovirology. 2010 Mar 12;7:18. [PMID: 20226045] 
Functional Description
 Gag-Pol polyprotein and Gag polyprotein may regulate their own translation, by the binding genomic RNA in the 5'-UTR. At low concentration, Gag-Pol and Gag would promote translation, whereas at high concentration, the polyproteins encapsidate genomic RNA and then shutt off translation. 
Sequence Annotation
 DOMAIN 508 577 Peptidase A2.
 DOMAIN 631 821 Reverse transcriptase.
 DOMAIN 1021 1144 RNase H.
 DOMAIN 1201 1351 Integrase catalytic.
 ZN_FING 390 407 CCHC-type 1.
 ZN_FING 411 428 CCHC-type 2.
 ZN_FING 1150 1191 Integrase-type.
 DNA_BIND 1370 1417 Integrase-type.
 REGION 217 225 PPIA/CYPA-binding loop.
 REGION 814 822 RT 'primer grip' (By similarity).
 MOTIF 16 22 Nuclear export signal (By similarity).
 MOTIF 26 32 Nuclear localization signal (By
 MOTIF 985 1001 Tryptophan repeat motif (By similarity).
 ACT_SITE 513 513 For protease activity; shared with
 METAL 697 697 Magnesium; catalytic; for reverse
 METAL 772 772 Magnesium; catalytic; for reverse
 METAL 773 773 Magnesium; catalytic; for reverse
 METAL 1030 1030 Magnesium; catalytic; for RNase H
 METAL 1065 1065 Magnesium; catalytic; for RNase H
 METAL 1085 1085 Magnesium; catalytic; for RNase H
 METAL 1136 1136 Magnesium; catalytic; for RNase H
 METAL 1211 1211 Magnesium; catalytic; for integrase
 METAL 1263 1263 Magnesium; catalytic; for integrase
 MOD_RES 132 132 Phosphotyrosine; by host (By similarity).
 LIPID 2 2 N-myristoyl glycine; by host (By  
Keyword
 3D-structure; Activation of host caspases by virus; AIDS; Aspartyl protease; Capsid maturation; Capsid protein; Complete proteome; DNA integration; DNA recombination; DNA-binding; DNA-directed DNA polymerase; Endonuclease; Host cell membrane; Host cytoplasm; Host gene expression shutoff by virus; Host membrane; Host nucleus; Host translation shutoff by virus; Host-virus interaction; Hydrolase; Lipoprotein; Magnesium; Membrane; Metal-binding; Modulation of host cell apoptosis by virus; Multifunctional enzyme; Myristate; Nuclease; Nucleotidyltransferase; Phosphoprotein; Protease; Reference proteome; Repeat; Ribosomal frameshifting; RNA-binding; RNA-directed DNA polymerase; Transferase; Viral genome integration; Viral nucleoprotein; Viral penetration into host nucleus; Virion; Virus entry into host cell; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1435 AA 
Protein Sequence
MGARASVLSG GELDRWEKIR LRPGGKKKYK LKHIVWASRE LERFAVNPGL LETSEGCRQI 60
LGQLQPSLQT GSEELRSLYN TVATLYCVHQ RIEIKDTKEA LDKIEEEQNK SKKKAQQAAA 120
DTGHSNQVSQ NYPIVQNIQG QMVHQAISPR TLNAWVKVVE EKAFSPEVIP MFSALSEGAT 180
PQDLNTMLNT VGGHQAAMQM LKETINEEAA EWDRVHPVHA GPIAPGQMRE PRGSDIAGTT 240
STLQEQIGWM TNNPPIPVGE IYKRWIILGL NKIVRMYSPT SILDIRQGPK EPFRDYVDRF 300
YKTLRAEQAS QEVKNWMTET LLVQNANPDC KTILKALGPA ATLEEMMTAC QGVGGPGHKA 360
RVLAEAMSQV TNSATIMMQR GNFRNQRKIV KCFNCGKEGH TARNCRAPRK KGCWKCGKEG 420
HQMKDCTERQ ANFLREDLAF LQGKAREFSS EQTRANSPTR RELQVWGRDN NSPSEAGADR 480
QGTVSFNFPQ VTLWQRPLVT IKIGGQLKEA LLDTGADDTV LEEMSLPGRW KPKMIGGIGG 540
FIKVRQYDQI LIEICGHKAI GTVLVGPTPV NIIGRNLLTQ IGCTLNFPIS PIETVPVKLK 600
PGMDGPKVKQ WPLTEEKIKA LVEICTEMEK EGKISKIGPE NPYNTPVFAI KKKDSTKWRK 660
LVDFRELNKR TQDFWEVQLG IPHPAGLKKK KSVTVLDVGD AYFSVPLDED FRKYTAFTIP 720
SINNETPGIR YQYNVLPQGW KGSPAIFQSS MTKILEPFRK QNPDIVIYQY MDDLYVGSDL 780
EIGQHRTKIE ELRQHLLRWG LTTPDKKHQK EPPFLWMGYE LHPDKWTVQP IVLPEKDSWT 840
VNDIQKLVGK LNWASQIYPG IKVRQLCKLL RGTKALTEVI PLTEEAELEL AENREILKEP 900
VHGVYYDPSK DLIAEIQKQG QGQWTYQIYQ EPFKNLKTGK YARMRGAHTN DVKQLTEAVQ 960
KITTESIVIW GKTPKFKLPI QKETWETWWT EYWQATWIPE WEFVNTPPLV KLWYQLEKEP 1020
IVGAETFYVD GAANRETKLG KAGYVTNRGR QKVVTLTDTT NQKTELQAIY LALQDSGLEV 1080
NIVTDSQYAL GIIQAQPDQS ESELVNQIIE QLIKKEKVYL AWVPAHKGIG GNEQVDKLVS 1140
AGIRKVLFLD GIDKAQDEHE KYHSNWRAMA SDFNLPPVVA KEIVASCDKC QLKGEAMHGQ 1200
VDCSPGIWQL DCTHLEGKVI LVAVHVASGY IEAEVIPAET GQETAYFLLK LAGRWPVKTI 1260
HTDNGSNFTG ATVRAACWWA GIKQEFGIPY NPQSQGVVES MNKELKKIIG QVRDQAEHLK 1320
TAVQMAVFIH NFKRKGGIGG YSAGERIVDI IATDIQTKEL QKQITKIQNF RVYYRDSRNP 1380
LWKGPAKLLW KGEGAVVIQD NSDIKVVPRR KAKIIRDYGK QMAGDDCVAS RQDED 1435 
Gene Ontology
 GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
 GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
 GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
 GO:0016020; C:membrane; IEA:UniProtKB-KW.
 GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
 GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
 GO:0004533; F:exoribonuclease H activity; IEA:EC.
 GO:0004523; F:ribonuclease H activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0003964; F:RNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
 GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
 GO:0030260; P:entry into host cell; TAS:Reactome.
 GO:0075713; P:establishment of integrated proviral latency; TAS:Reactome.
 GO:0039651; P:induction by virus of host cysteine-type endopeptidase activity involved in apoptotic process; IEA:UniProtKB-KW.
 GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0006278; P:RNA-dependent DNA replication; TAS:Reactome.
 GO:0019061; P:uncoating of virus; TAS:Reactome.
 GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
 GO:0075732; P:viral penetration into host nucleus; IEA:UniProtKB-KW.
 GO:0046797; P:viral procapsid maturation; IEA:UniProtKB-KW. 
Interpro
 IPR000721; Gag_p24.
 IPR001037; Integrase_C_retrovir.
 IPR001584; Integrase_cat-core.
 IPR017856; Integrase_Zn-bd_dom-like_N.
 IPR003308; Integrase_Zn-bd_dom_N.
 IPR000071; Lentvrl_matrix_N.
 IPR012344; Matrix_N_HIV/RSV.
 IPR018061; Pept_A2A_retrovirus_sg.
 IPR001995; Peptidase_A2_cat.
 IPR021109; Peptidase_aspartic.
 IPR001969; Peptidase_aspartic_AS.
 IPR008916; Retrov_capsid_C.
 IPR008919; Retrov_capsid_N.
 IPR010999; Retrovr_matrix_N.
 IPR012337; RNaseH-like_dom.
 IPR002156; RNaseH_domain.
 IPR000477; RVT.
 IPR010659; RVT_connect.
 IPR010661; RVT_thumb.
 IPR001878; Znf_CCHC. 
Pfam
 PF00540; Gag_p17
 PF00607; Gag_p24
 PF00552; IN_DBD_C
 PF02022; Integrase_Zn
 PF00075; RNase_H
 PF00665; rve
 PF00077; RVP
 PF00078; RVT_1
 PF06815; RVT_connect
 PF06817; RVT_thumb
 PF00098; zf-CCHC 
SMART
 SM00343; ZnF_C2HC 
PROSITE
 PS50175; ASP_PROT_RETROV
 PS00141; ASP_PROTEASE
 PS50994; INTEGRASE
 PS51027; INTEGRASE_DBD
 PS50879; RNASE_H
 PS50878; RT_POL
 PS50158; ZF_CCHC
 PS50876; ZF_INTEGRASE 
PRINTS
 PR00234; HIV1MATRIX.