CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012412
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Importin subunit beta-1 
Protein Synonyms/Alias
 Importin-90; Karyopherin subunit beta-1; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97 
Gene Name
 KPNB1 
Gene Synonyms/Alias
 NTF97 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
23LELEAAQKFLERAAVubiquitination[1, 2, 3, 4, 5, 6]
62VAAGLQIKNSLTSKDubiquitination[1, 4, 6]
68IKNSLTSKDPDIKAQubiquitination[2, 4]
73TSKDPDIKAQYQQRWubiquitination[4]
191EEPSNNVKLAATNALubiquitination[4]
206LNSLEFTKANFDKESubiquitination[1, 2, 4, 6, 7]
211FTKANFDKESERHFIacetylation[8]
211FTKANFDKESERHFIubiquitination[2, 4, 6, 7]
376PFIKEHIKNPDWRYRubiquitination[1, 2, 4, 5, 6]
537ESLMEIVKNSAKDCYubiquitination[1, 2, 4, 5, 6, 9]
541EIVKNSAKDCYPAVQubiquitination[2, 4]
549DCYPAVQKTTLVIMEubiquitination[2, 4]
594TLQNVLRKVQHQDALubiquitination[4]
651LKYMEAFKPFLGIGLubiquitination[1, 2, 6]
710ENVHRSVKPQILSVFubiquitination[2]
730AIGGEFKKYLEVVLNubiquitination[1, 2, 3, 6, 9]
777TGIVQGLKGDQENVHubiquitination[2, 4]
835AFGKDVLKLVEARPMacetylation[8]
835AFGKDVLKLVEARPMubiquitination[1, 6]
859RSKTNKAKTLATWATubiquitination[4]
867TLATWATKELRKLKNacetylation[8]
867TLATWATKELRKLKNubiquitination[1, 4, 6]
871WATKELRKLKNQA**ubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
 Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.
 Proteomics. 2007 Mar;7(6):868-74. [PMID: 17370265]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [9] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865] 
Functional Description
 Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re- exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus. 
Sequence Annotation
 DOMAIN 21 101 Importin N-terminal.
 REPEAT 124 163 HEAT 1.
 REPEAT 168 207 HEAT 2.
 REPEAT 213 250 HEAT 3.
 REPEAT 317 357 HEAT 4.
 REPEAT 362 399 HEAT 5.
 REPEAT 404 441 HEAT 6.
 REPEAT 602 641 HEAT 7.
 REPEAT 687 726 HEAT 8.
 REGION 329 342 IAB-binding.
 REGION 334 419 Ran-GTP binding.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 12 12 Phosphoserine.
 MOD_RES 211 211 N6-acetyllysine; alternate.
 MOD_RES 835 835 N6-acetyllysine.
 MOD_RES 867 867 N6-acetyllysine.
 CROSSLNK 206 206 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 211 211 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; ADP-ribosylation; Complete proteome; Cytoplasm; Direct protein sequencing; Host-virus interaction; Isopeptide bond; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 876 AA 
Protein Sequence
MELITILEKT VSPDRLELEA AQKFLERAAV ENLPTFLVEL SRVLANPGNS QVARVAAGLQ 60
IKNSLTSKDP DIKAQYQQRW LAIDANARRE VKNYVLQTLG TETYRPSSAS QCVAGIACAE 120
IPVNQWPELI PQLVANVTNP NSTEHMKEST LEAIGYICQD IDPEQLQDKS NEILTAIIQG 180
MRKEEPSNNV KLAATNALLN SLEFTKANFD KESERHFIMQ VVCEATQCPD TRVRVAALQN 240
LVKIMSLYYQ YMETYMGPAL FAITIEAMKS DIDEVALQGI EFWSNVCDEE MDLAIEASEA 300
AEQGRPPEHT SKFYAKGALQ YLVPILTQTL TKQDENDDDD DWNPCKAAGV CLMLLATCCE 360
DDIVPHVLPF IKEHIKNPDW RYRDAAVMAF GCILEGPEPS QLKPLVIQAM PTLIELMKDP 420
SVVVRDTAAW TVGRICELLP EAAINDVYLA PLLQCLIEGL SAEPRVASNV CWAFSSLAEA 480
AYEAADVADD QEEPATYCLS SSFELIVQKL LETTDRPDGH QNNLRSSAYE SLMEIVKNSA 540
KDCYPAVQKT TLVIMERLQQ VLQMESHIQS TSDRIQFNDL QSLLCATLQN VLRKVQHQDA 600
LQISDVVMAS LLRMFQSTAG SGGVQEDALM AVSTLVEVLG GEFLKYMEAF KPFLGIGLKN 660
YAEYQVCLAA VGLVGDLCRA LQSNIIPFCD EVMQLLLENL GNENVHRSVK PQILSVFGDI 720
ALAIGGEFKK YLEVVLNTLQ QASQAQVDKS DYDMVDYLNE LRESCLEAYT GIVQGLKGDQ 780
ENVHPDVMLV QPRVEFILSF IDHIAGDEDH TDGVVACAAG LIGDLCTAFG KDVLKLVEAR 840
PMIHELLTEG RRSKTNKAKT LATWATKELR KLKNQA 876 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0005643; C:nuclear pore; TAS:ProtInc.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0008139; F:nuclear localization sequence binding; TAS:ProtInc.
 GO:0008565; F:protein transporter activity; IEA:Compara.
 GO:0008270; F:zinc ion binding; TAS:ProtInc.
 GO:0006309; P:apoptotic DNA fragmentation; TAS:Reactome.
 GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
 GO:0075733; P:intracellular transport of viral material; TAS:Reactome.
 GO:0006607; P:NLS-bearing substrate import into nucleus; TAS:ProtInc.
 GO:0000060; P:protein import into nucleus, translocation; TAS:ProtInc.
 GO:0006610; P:ribosomal protein import into nucleus; IDA:UniProtKB.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0019058; P:viral infectious cycle; TAS:Reactome. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000225; Armadillo.
 IPR021133; HEAT_type_2.
 IPR001494; Importin-beta_N.
 IPR027140; KPNB1. 
Pfam
 PF00514; Arm
 PF03810; IBN_N 
SMART
 SM00913; IBN_N 
PROSITE
 PS50077; HEAT_REPEAT
 PS50166; IMPORTIN_B_NT 
PRINTS