CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022331
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DDB1- and CUL4-associated factor 16 
Protein Synonyms/Alias
  
Gene Name
 DCAF16 
Gene Synonyms/Alias
 C4orf30 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
61WQVKCLLKYSTTWKPacetylation[1]
79NSWLYHAKLLDPSTPubiquitination[2]
150GALQFATKQLSRTLSubiquitination[2, 3, 4, 5, 6, 7]
167TPIPEYLKQIPNSCVubiquitination[5, 6]
206YSYTDFQKAVNKLLTubiquitination[4]
210DFQKAVNKLLTASL*ubiquitination[6]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. 
Sequence Annotation
 MOD_RES 61 61 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Polymorphism; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 216 AA 
Protein Sequence
MGPRNPSPDH LSESESEEEE NISYLNESSG EEWDSSEEED SMVPNLSPLE SLAWQVKCLL 60
KYSTTWKPLN PNSWLYHAKL LDPSTPVHIL REIGLRLSHC SHCVPKLEPI PEWPPLASCG 120
VPPFQKPLTS PSRLSRDHAT LNGALQFATK QLSRTLSRAT PIPEYLKQIP NSCVSGCCCG 180
WLTKTVKETT RTEPINTTYS YTDFQKAVNK LLTASL 216 
Gene Ontology
 GO:0080008; C:Cul4-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0016567; P:protein ubiquitination; IC:UniProtKB. 
Interpro
  
Pfam
  
SMART
  
PROSITE
  
PRINTS