CPLM-004534

Genbank Nucleotide ID

High mobility group protein HMG-I/HMG-Y

Protein Synonyms/Alias

HMG-I(Y); High mobility group AT-hook protein 1; High mobility group protein A1

Mus musculus (Mouse)

Position	Peptide	Type	References
7	*MSESGSKSSQPLAS	acetylation	[1]
7	*MSESGSKSSQPLAS	succinylation	[1]
7	*MSESGSKSSQPLAS	ubiquitination	[2]
15	SSQPLASKQEKDGTE	acetylation	[1, 3, 4, 5, 6, 7, 8]
31	RGRGRPRKQPPVSPG	acetylation	[1]
46	TALVGSQKEPSEVPT	acetylation	[1]

[1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[3] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
[4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[5] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[6] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[8] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]

Functional Description

HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.

DNA_BIND 21 31 A.T hook 1.
DNA_BIND 53 63 A.T hook 2.
DNA_BIND 78 89 A.T hook 3.
REGION 53 77 Interaction with HIPK2.
MOD_RES 2 2 N-acetylserine; in isoform HMG-Y.
MOD_RES 15 15 N6-acetyllysine (By similarity).
MOD_RES 26 26 Asymmetric dimethylarginine; alternate
MOD_RES 26 26 Omega-N-methylarginine; alternate (By
MOD_RES 26 26 Symmetric dimethylarginine; alternate (By
MOD_RES 36 36 Phosphoserine; by HIPK2 and CDC2; in
MOD_RES 39 39 Phosphothreonine (By similarity).
MOD_RES 44 44 Phosphoserine.
MOD_RES 49 49 Phosphoserine; in isoform HMG-I.
MOD_RES 53 53 Phosphothreonine; by HIPK2 and CDC2; in
MOD_RES 58 58 Asymmetric dimethylarginine; by PRMT6;
MOD_RES 58 58 Omega-N-methylarginine; by PRMT6;
MOD_RES 60 60 Asymmetric dimethylarginine; by PRMT6;
MOD_RES 60 60 Omega-N-methylarginine; by PRMT6;
MOD_RES 99 99 Phosphoserine; in isoform HMG-Y.
MOD_RES 102 102 Phosphoserine; in isoform HMG-Y.
MOD_RES 103 103 Phosphoserine; in isoform HMG-Y.

Acetylation; Alternative splicing; Chromosome; Complete proteome; DNA-binding; Methylation; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005634; C:nucleus; ISS:UniProtKB.
GO:0035985; C:senescence-associated heterochromatin focus; ISS:UniProtKB.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
GO:0030374; F:ligand-dependent nuclear receptor transcription coactivator activity; ISS:UniProtKB.
GO:0042975; F:peroxisome proliferator activated receptor binding; ISS:UniProtKB.
GO:0046965; F:retinoid X receptor binding; ISS:UniProtKB.
GO:0003700; F:sequence-specific DNA binding transcription factor activity; ISS:UniProtKB.
GO:0008285; P:negative regulation of cell proliferation; ISS:UniProtKB.
GO:0045892; P:negative regulation of transcription, DNA-dependent; ISS:UniProtKB.
GO:0090402; P:oncogene-induced senescence; ISS:UniProtKB.
GO:2000774; P:positive regulation of cellular senescence; ISS:UniProtKB.
GO:0045893; P:positive regulation of transcription, DNA-dependent; ISS:UniProtKB.
GO:0035986; P:senescence-associated heterochromatin focus assembly; ISS:UniProtKB.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR020478; AT_hook-like.
IPR017956; AT_hook_DNA-bd_motif.
IPR000116; HMGI/HMGY.
IPR000637; HMGI/Y_DNA-bd_CS.

PR00929; ATHOOK.
PR00930; HIGHMOBLTYIY.