CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001741
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Malate dehydrogenase, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 MDH2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Sus scrofa (Pig) 
NCBI Taxa ID
 9823 
Lysine Modification
Position
Peptide
Type
References
239EAGTEVVKAKAGAGSmalonylation[1]
239EAGTEVVKAKAGAGSsuccinylation[1]
301LGKKGIEKNLGIGKIsuccinylation[1]
328PELKASIKKGEEFVKsuccinylation[1]
329ELKASIKKGEEFVKNmalonylation[1]
Reference
 [1] Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase.
 Du J, Zhou Y, Su X, Yu JJ, Khan S, Jiang H, Kim J, Woo J, Kim JH, Choi BH, He B, Chen W, Zhang S, Cerione RA, Auwerx J, Hao Q, Lin H.
 Science. 2011 Nov 11;334(6057):806-9. [PMID: 22076378] 
Functional Description
  
Sequence Annotation
 NP_BIND 31 37 NAD (By similarity).
 NP_BIND 140 142 NAD (By similarity).
 ACT_SITE 200 200 Proton acceptor.
 BINDING 57 57 NAD (By similarity).
 BINDING 104 104 Substrate.
 BINDING 110 110 Substrate.
 BINDING 117 117 NAD (By similarity).
 BINDING 142 142 Substrate.
 BINDING 176 176 Substrate.
 BINDING 251 251 NAD (By similarity).
 MOD_RES 157 157 N6-acetyllysine (By similarity).
 MOD_RES 165 165 N6-acetyllysine (By similarity).
 MOD_RES 185 185 N6-acetyllysine (By similarity).
 MOD_RES 239 239 N6-malonyllysine; alternate (By
 MOD_RES 239 239 N6-succinyllysine; alternate (By
 MOD_RES 301 301 N6-acetyllysine; alternate (By
 MOD_RES 301 301 N6-succinyllysine; alternate (By
 MOD_RES 307 307 N6-acetyllysine; alternate (By
 MOD_RES 307 307 N6-malonyllysine; alternate (By
 MOD_RES 314 314 N6-acetyllysine (By similarity).
 MOD_RES 328 328 N6-acetyllysine; alternate (By
 MOD_RES 328 328 N6-succinyllysine; alternate (By
 MOD_RES 329 329 N6-acetyllysine; alternate (By
 MOD_RES 329 329 N6-malonyllysine; alternate (By
 MOD_RES 335 335 N6-acetyllysine (By similarity).  
Keyword
 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide; Tricarboxylic acid cycle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 338 AA 
Protein Sequence
MLSALARPAG AALRRSFSTS XQNNAKVAVL GASGGIGQPL SLLLKNSPLV SRLTLYDIAH 60
TPGVAADLSH IETRATVKGY LGPEQLPDCL KGCDVVVIPA GVPRKPGMTR DDLFNTNATI 120
VATLTAACAQ HCPDAMICII SNPVNSTIPI TAEVFKKHGV YNPNKIFGVT TLDIVRANAF 180
VAELKGLDPA RVSVPVIGGH AGKTIIPLIS QCTPKVDFPQ DQLSTLTGRI QEAGTEVVKA 240
KAGAGSATLS MAYAGARFVF SLVDAMNGKE GVVECSFVKS QETDCPYFST PLLLGKKGIE 300
KNLGIGKISP FEEKMIAEAI PELKASIKKG EEFVKNMK 338 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0030060; F:L-malate dehydrogenase activity; IEA:EC.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006475; P:internal protein amino acid acetylation; ISS:UniProtKB.
 GO:0006108; P:malate metabolic process; IEA:InterPro.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW. 
Interpro
 IPR001557; L-lactate/malate_DH.
 IPR022383; Lactate/malate_DH_C.
 IPR001236; Lactate/malate_DH_N.
 IPR015955; Lactate_DH/Glyco_Ohase_4_C.
 IPR001252; Malate_DH_AS.
 IPR010097; Malate_DH_type1.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF02866; Ldh_1_C
 PF00056; Ldh_1_N 
SMART
  
PROSITE
 PS00068; MDH 
PRINTS