CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010655
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Unconventional myosin-VIIa 
Protein Synonyms/Alias
  
Gene Name
 Myo7a 
Gene Synonyms/Alias
 Myo7 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
768RSNFLRLKSAATLIQubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the retina, plays an important role in the renewal of the outer photoreceptor disks. Plays an important role in the distribution and migration of retinal pigment epithelial (RPE) melanosomes and phagosomes, and in the regulation of opsin transport in retinal photoreceptors. Mediates intracellular transport of RPE65 in the retina pigment epithelium. In the inner ear, plays an important role in differentiation, morphogenesis and organization of cochlear hair cell bundles. Motor protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity. 
Sequence Annotation
 DOMAIN 1 729 Myosin head-like.
 DOMAIN 745 765 IQ 1.
 DOMAIN 768 788 IQ 2.
 DOMAIN 791 811 IQ 3.
 DOMAIN 814 834 IQ 4.
 DOMAIN 837 857 IQ 5.
 DOMAIN 1017 1253 MyTH4 1.
 DOMAIN 1258 1602 FERM 1.
 DOMAIN 1603 1672 SH3.
 DOMAIN 1747 1896 MyTH4 2.
 DOMAIN 1902 2205 FERM 2.
 NP_BIND 158 165 ATP (Probable).
 REGION 632 639 Actin-binding (Probable).
 MOD_RES 965 965 Phosphoserine.  
Keyword
 3D-structure; Actin-binding; Alternative splicing; ATP-binding; Calmodulin-binding; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Deafness; Disease mutation; Hearing; Motor protein; Myosin; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; SH3 domain; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2215 AA 
Protein Sequence
MVILQKGDYV WMDLKSGQEF DVPIGAVVKL CDSGQIQVVD DEDNEHWISP QNATHIKPMH 60
PTSVHGVEDM IRLGDLNEAG ILRNLLIRYR DHLIYTYTGS ILVAVNPYQL LSIYSPEHIR 120
QYTNKKIGEM PPHIFAIADN CYFNMKRNNR DQCCIISGES GAGKTESTKL ILQFLAAISG 180
QHSWIEQQVL EATPILEAFG NAKTIRNDNS SRFGKYIDIH FNKRGAIEGA KIEQYLLEKS 240
RVCRQAPDER NYHVFYCMLE GMNEEEKKKL GLGQAADYNY LAMGNCITCE GRVDSQEYAN 300
IRSAMKVLMF TDTENWEISK LLAAILHMGN LQYEARTFEN LDACEVLFSP SLATAASLLE 360
VNPPDLMSCL TSRTLITRGE TVSTPLSREQ ALDVRDAFVK GIYGRLFVWI VEKINAAIYK 420
PPPLEVKNSR RSIGLLDIFG FENFTVNSFE QLCINFANEH LQQFFVRHVF KLEQEEYDLE 480
SIDWLHIEFT DNQEALDMIA NRPMNVISLI DEESKFPKGT DATMLHKLNS QHKLNANYVP 540
PKNSHETQFG INHFAGVVYY ESQGFLEKNR DTLHGDIIQL VHSSRNKFIK QIFQADVAMG 600
AETRKRSPTL SSQFKRSLEL LMRTLGACQP FFVRCIKPNE FKKPMLFDRH LCVRQLRYSG 660
MMETIRIRHA GYPIRYSFVE FVERYRVLLP GVKPAYKQGD LRGTCQRMAE AVLGTHDDWQ 720
IGKTKIFLKD HHDMLLEVER DKAITDRVIL LQKVIRGFKD RSNFLRLKSA ATLIQRHWRG 780
HHCRKNYELI RLGFLRLQAL HRSRKLHKQY RLARQRIIEF QARCRAYLVR KAFRHRLWAV 840
ITVQAYARGM IARRLHRRLR VEYQRRLEAE RMRLAEEEKL RKEMSAKKAK EEAERKHQER 900
LAQLAREDAE RELKEKEEAR RKKELLEQME KARHEPINHS DMVDKMFGFL GTSGSLPGQE 960
GQAPSGFEDL ERGRREMVEE DVDAALPLPD EDEEDLSEYK FAKFAATYFQ GTTTHSYTRR 1020
PLKQPLLYHD DEGDQLAALA VWITILRFMG DLPEPKYHTA MSDGSEKIPV MTKIYETLGK 1080
KTYKRELQAL QGEGETQLPE GQKKTSVRHK LVHLTLKKKS KLTEEVTKRL NDGESTVQGN 1140
SMLEDRPTSN LEKLHFIIGN GILRPALRDE IYCQISKQLT HNPSKSSYAR GWILVSLCVG 1200
CFAPSEKFVK YLRNFIHGGP PGYAPYCEER LRRTFVNGTR TQPPSWLELQ ATKSKKPIML 1260
PVTFMDGTTK TLLTDSATTA RELCNALADK ISLKDRFGFS LYIALFDKVS SLGSGSDHVM 1320
DAISQCEQYA KEQGAQERNA PWRLFFRKEV FTPWHNPSED NVATNLIYQQ VVRGVKFGEY 1380
RCEKEDDLAE LASQQYFVDY GSEMILERLL SLVPTYIPDR EITPLKNLEK WAQLAIAAHK 1440
KGIYAQRRTD SQKVKEDVVN YARFKWPLLF SRFYEAYKFS GPPLPKSDVI VAVNWTGVYF 1500
VDEQEQVLLE LSFPEIMAVS SSRECRVLLS LGCSDLGCAT CQSGRAGLTP AGPCSPCWSC 1560
RGTKMMAPSF TLATIKGDEY TFTSSNAEDI RDLVVTFLEG LRKRSKYVVA LQDNPNPAGE 1620
ESGFLSFAKG DLIILDHDTG EQVMNSGWAN GINERTKQRG DFPTDCVYVM PTVTLPPREI 1680
VALVTMTPDQ RQDVVRLLQL RTAEPEVRAK PYTLEEFSYD YFRPPPKHTL SRVMVSKARG 1740
KDRLWSHTRE PLKQALLKKI LGSEELSQEA CMAFVAVLKY MGDYPSKRMR SVNELTDQIF 1800
EWALKAEPLK DEAYVQILKQ LTDNHIRYSE ERGWELLWLC TGLFPPSNIL LPHVQRFLQS 1860
RKHCPLAIDC LQRLQKALRN GSRKYPPHLV EVEAIQHKTT QIFHKVYFPD DTDEAFEVES 1920
STKAKDFCQN IASRLLLKSS EGFSLFVKIA DKVISVPEND FFFDFVRHLT DWIKKARPIK 1980
DGIVPSLTYQ VFFMKKLWTT TVPGKDPMAD SIFHYYQELP KYLRGYHKCT REEVLQLGAL 2040
IYRVKFEEDK SYFPSIPKLL RELVPQDLIR QVSPDDWKRS IVAYFNKHAG KSKEEAKLAF 2100
LKLIFKWPTF GSAFFEVKQT TEPNFPEILL IAINKYGVSL IDPRTKDILT THPFTKISNW 2160
SSGNTYFHIT IGNLVRGSKL LCETSLGYKM DDLLTSYISQ MLTAMSKQRN SRSGR 2215 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IDA:MGI.
 GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0005765; C:lysosomal membrane; ISS:UniProtKB.
 GO:0042470; C:melanosome; IDA:MGI.
 GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
 GO:0032391; C:photoreceptor connecting cilium; IDA:MGI.
 GO:0001917; C:photoreceptor inner segment; ISS:UniProtKB.
 GO:0001750; C:photoreceptor outer segment; ISS:UniProtKB.
 GO:0032420; C:stereocilium; IDA:MGI.
 GO:0045202; C:synapse; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005516; F:calmodulin binding; ISS:UniProtKB.
 GO:0000146; F:microfilament motor activity; IEA:Compara.
 GO:0030048; P:actin filament-based movement; IEA:Compara.
 GO:0060088; P:auditory receptor cell stereocilium organization; IMP:MGI.
 GO:0050957; P:equilibrioception; IEA:Compara.
 GO:0006886; P:intracellular protein transport; IMP:MGI.
 GO:0007040; P:lysosome organization; ISS:UniProtKB.
 GO:0001845; P:phagolysosome assembly; IMP:MGI.
 GO:0051904; P:pigment granule transport; IMP:MGI.
 GO:0048563; P:post-embryonic organ morphogenesis; IMP:MGI.
 GO:0007605; P:sensory perception of sound; ISS:UniProtKB.
 GO:0007601; P:visual perception; ISS:UniProtKB. 
Interpro
 IPR019749; Band_41_domain.
 IPR014352; FERM/acyl-CoA-bd_prot_3-hlx.
 IPR019748; FERM_central.
 IPR000299; FERM_domain.
 IPR018979; FERM_N.
 IPR000048; IQ_motif_EF-hand-BS.
 IPR001609; Myosin_head_motor_dom.
 IPR000857; MyTH4_dom.
 IPR027417; P-loop_NTPase.
 IPR011993; PH_like_dom.
 IPR001452; SH3_domain. 
Pfam
 PF00373; FERM_M
 PF09379; FERM_N
 PF00612; IQ
 PF00063; Myosin_head
 PF00784; MyTH4 
SMART
 SM00295; B41
 SM00015; IQ
 SM00242; MYSc
 SM00139; MyTH4
 SM00326; SH3 
PROSITE
 PS00660; FERM_1
 PS00661; FERM_2
 PS50057; FERM_3
 PS50096; IQ
 PS51016; MYTH4
 PS50002; SH3 
PRINTS
 PR00193; MYOSINHEAVY.