CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008199
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dual specificity protein kinase CLK3 
Protein Synonyms/Alias
 CDC-like kinase 3 
Gene Name
 CLK3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
288SRSVEDDKEGHLVCRubiquitination[1]
317LGEGTFGKVVECLDHubiquitination[1]
328CLDHARGKSQVALKIubiquitination[1]
334GKSQVALKIIRNVGKubiquitination[1]
354RLEINVLKKIKEKDKubiquitination[1]
396KNTFEFLKENNFQPYubiquitination[1]
553IHRTRKQKYFYKGGLubiquitination[1]
557RKQKYFYKGGLVWDEubiquitination[1, 2]
577RYVKENCKPLKSYMLubiquitination[1]
580KENCKPLKSYMLQDSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Phosphorylates SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. 
Sequence Annotation
 DOMAIN 304 620 Protein kinase.
 NP_BIND 310 318 ATP (By similarity).
 ACT_SITE 431 431 Proton acceptor (By similarity).
 BINDING 334 334 ATP (By similarity).
 MOD_RES 155 155 Phosphotyrosine.
 MOD_RES 157 157 Phosphoserine.
 MOD_RES 215 215 Phosphoserine.
 MOD_RES 224 224 Phosphoserine.
 MOD_RES 226 226 Phosphoserine.
 MOD_RES 283 283 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 638 AA 
Protein Sequence
MPVLSARRRE LADHAGSGRR SGPSPTARSG PHLSALRAQP ARAAHLSGRG TYVRRDTAGG 60
GPGQARPLGP PGTSLLGRGA RRSGEGWCPG AFESGARAAR PPSRVEPRLA TAASREGAGL 120
PRAEVAAGSG RGARSGEWGL AAAGAWETMH HCKRYRSPEP DPYLSYRWKR RRSYSREHEG 180
RLRYPSRREP PPRRSRSRSH DRLPYQRRYR ERRDSDTYRC EERSPSFGED YYGPSRSRHR 240
RRSRERGPYR TRKHAHHCHK RRTRSCSSAS SRSQQSSKRS SRSVEDDKEG HLVCRIGDWL 300
QERYEIVGNL GEGTFGKVVE CLDHARGKSQ VALKIIRNVG KYREAARLEI NVLKKIKEKD 360
KENKFLCVLM SDWFNFHGHM CIAFELLGKN TFEFLKENNF QPYPLPHVRH MAYQLCHALR 420
FLHENQLTHT DLKPENILFV NSEFETLYNE HKSCEEKSVK NTSIRVADFG SATFDHEHHT 480
TIVATRHYRP PEVILELGWA QPCDVWSIGC ILFEYYRGFT LFQTHENREH LVMMEKILGP 540
IPSHMIHRTR KQKYFYKGGL VWDENSSDGR YVKENCKPLK SYMLQDSLEH VQLFDLMRRM 600
LEFDPAQRIT LAEALLHPFF AGLTPEERSF HTSRNPSR 638 
Gene Ontology
 GO:0001669; C:acrosomal vesicle; IEA:UniProtKB-SubCell.
 GO:0045111; C:intermediate filament cytoskeleton; IDA:HPA.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; ISS:UniProtKB.
 GO:0004712; F:protein serine/threonine/tyrosine kinase activity; IEA:EC.
 GO:0004713; F:protein tyrosine kinase activity; IEA:UniProtKB-KW.
 GO:0018108; P:peptidyl-tyrosine phosphorylation; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; IEA:Compara.
 GO:0043484; P:regulation of RNA splicing; IDA:UniProtKB. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS