CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004164
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NADP-dependent malic enzyme 
Protein Synonyms/Alias
 NADP-ME; Malic enzyme 1 
Gene Name
 Me1 
Gene Synonyms/Alias
 Mod-1; Mod1 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
516VSLKIAVKIVQDAYKubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
  
Sequence Annotation
 NP_BIND 301 318 NADP (By similarity).
 ACT_SITE 102 102 Proton donor (By similarity).
 ACT_SITE 173 173 Proton acceptor (By similarity).
 METAL 245 245 Divalent metal cation (By similarity).
 METAL 246 246 Divalent metal cation (By similarity).
 METAL 269 269 Divalent metal cation (By similarity).
 BINDING 155 155 NADP (By similarity).
 BINDING 269 269 NADP (By similarity).
 BINDING 408 408 NADP (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Metal-binding; NADP; Oxidoreductase; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 572 AA 
Protein Sequence
MDPRAPRRRH THQRGYLLTR DPHLNKDLAF TLEERQQLKI HGLLPPCIVN QEIQVLRVIK 60
NFERLNSDFD RYLLLMDLQD RNEKLFYSVL MSNVEKFMPI VYTPTVGLAC QQYSLAFRKP 120
RGLFISIHDK GHIASVLNAW PEDVVKAIVV TDGERILGLG DLGCNGMGIP VGKLALYTAC 180
GGVNPQQCLP ITLDVGTENE ELLKDPLYIG LRHRRVRGPE YDAFLDEFME AASSKYGMNC 240
LIQFEDFANL NAFRLLNKYR NKYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTVLFQ 300
GAGEAALGIA HLIVMAMEKE GLSKEKARQK IWLVDSKGLI VKGRASLTEE KEVFAHEHEE 360
MKNLEAIVQK IKPTALIGVA AIGGAFTEQI LKDMAAFNER PIIFALSNPT SKAECSAEEC 420
YKVTKGRAIF ASGSPFDPVT LPDGRTLFPG QGNNSYVFPG VALGVVACGL RHINDSVFLT 480
TAEVISQQVS DKHLEEGRLY PPLNTIRDVS LKIAVKIVQD AYKEKMATVY PEPQNKEEFV 540
SSQMYSTNYD QILPDCYSWP EEVQKIQTKV NQ 572 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0004473; F:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; IDA:UniProtKB.
 GO:0016619; F:malate dehydrogenase (oxaloacetate-decarboxylating) activity; IEA:InterPro.
 GO:0030145; F:manganese ion binding; ISS:UniProtKB.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0050661; F:NADP binding; IDA:RGD.
 GO:0006108; P:malate metabolic process; ISS:UniProtKB.
 GO:0009725; P:response to hormone stimulus; IDA:UniProtKB. 
Interpro
 IPR015884; Malic_enzyme_CS.
 IPR012301; Malic_N.
 IPR012302; Malic_NAD-bd.
 IPR001891; Malic_OxRdtase.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00390; malic
 PF03949; Malic_M 
SMART
 SM00919; Malic_M 
PROSITE
 PS00331; MALIC_ENZYMES 
PRINTS
 PR00072; MALOXRDTASE.