CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003813
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lanosterol 14-alpha demethylase 
Protein Synonyms/Alias
 CYPLI; Cytochrome P450 51; Cytochrome P450-14DM; Cytochrome P450-LIA1; Sterol 14-alpha demethylase 
Gene Name
 ERG11 
Gene Synonyms/Alias
 CYP51; YHR007C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
107MTVYLGPKGHEFVFNubiquitination[1]
116HEFVFNAKLADVSAEubiquitination[1, 2]
136LTTPVFGKGVIYDCPubiquitination[1]
155MEQKKFVKGALTKEAubiquitination[1]
160FVKGALTKEAFKSYVubiquitination[1]
164ALTKEAFKSYVPLIAubiquitination[1]
176LIAEEVYKYFRDSKNubiquitination[1]
215ASRSLLGKEMRAKLDubiquitination[1]
270GTYMSLIKERRKNNDubiquitination[1]
294LMKNSTYKDGVKMTDubiquitination[1]
353RVLDGGKKELTYDLLubiquitination[2]
371PLLNQTIKETLRMHHubiquitination[1, 2]
433FNIHRWNKDSASSYSubiquitination[1]
454YGFGAISKGVSSPYLubiquitination[1, 2]
529EKRNPEQKI******ubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. 
Sequence Annotation
 METAL 470 470 Iron (heme axial ligand) (By similarity).
 MOD_RES 458 458 Phosphoserine.  
Keyword
 Complete proteome; Heme; Iron; Lipid biosynthesis; Lipid metabolism; Membrane; Metal-binding; Monooxygenase; NADP; Oxidoreductase; Phosphoprotein; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 530 AA 
Protein Sequence
MSATKSIVGE ALEYVNIGLS HFLALPLAQR ISLIIIIPFI YNIVWQLLYS LRKDRPPLVF 60
YWIPWVGSAV VYGMKPYEFF EECQKKYGDI FSFVLLGRVM TVYLGPKGHE FVFNAKLADV 120
SAEAAYAHLT TPVFGKGVIY DCPNSRLMEQ KKFVKGALTK EAFKSYVPLI AEEVYKYFRD 180
SKNFRLNERT TGTIDVMVTQ PEMTIFTASR SLLGKEMRAK LDTDFAYLYS DLDKGFTPIN 240
FVFPNLPLEH YRKRDHAQKA ISGTYMSLIK ERRKNNDIQD RDLIDSLMKN STYKDGVKMT 300
DQEIANLLIG VLMGGQHTSA ATSAWILLHL AERPDVQQEL YEEQMRVLDG GKKELTYDLL 360
QEMPLLNQTI KETLRMHHPL HSLFRKVMKD MHVPNTSYVI PAGYHVLVSP GYTHLRDEYF 420
PNAHQFNIHR WNKDSASSYS VGEEVDYGFG AISKGVSSPY LPFGGGRHRC IGEHFAYCQL 480
GVLMSIFIRT LKWHYPEGKT VPPPDFTSMV TLPTGPAKII WEKRNPEQKI 530 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:SGD.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0008398; F:sterol 14-demethylase activity; IDA:SGD.
 GO:0006696; P:ergosterol biosynthetic process; IMP:SGD. 
Interpro
 IPR001128; Cyt_P450.
 IPR017972; Cyt_P450_CS.
 IPR002403; Cyt_P450_E_grp-IV. 
Pfam
 PF00067; p450 
SMART
  
PROSITE
 PS00086; CYTOCHROME_P450 
PRINTS
 PR00465; EP450IV.
 PR00385; P450.