UniProt Accession

 ARF1_YEAST; P11076; D6VRG1 

Genbank Protein ID

 J03276; X83276; Z74240; BK006938 

Genbank Nucleotide ID

 AAA34431.1; CAA58255.1; CAA98769.1; DAA11671.1 

Protein Name

 ADP-ribosylation factor 1 

Protein Synonyms/Alias

  

Gene Name

 ARF1 

Gene Synonyms/Alias

 YDL192W; D1244 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
16	FSNLFGNKEMRILMV	acetylation	[1]
16	FSNLFGNKEMRILMV	ubiquitination	[2]
36	GKTTVLYKLKLGEVI	acetylation	[1]
127	AWLVFANKQDLPEAM	ubiquitination	[2, 3]
142	SAAEITEKLGLHSIR	acetylation	[1]
142	SAAEITEKLGLHSIR	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recruits polyadenylate-binding protein PAB1 to COPI vesicles, and this is required for correct localization of the asymmetrically distributed ASH1 mRNA. 

Sequence Annotation

 NP_BIND 25 32 GTP.
 NP_BIND 126 129 GTP.
 NP_BIND 160 161 GTP.
 BINDING 48 48 GTP.
 BINDING 70 70 GTP; via amide nitrogen.
 LIPID 2 2 N-myristoyl glycine.  

Keyword

 3D-structure; Complete proteome; ER-Golgi transport; Golgi apparatus; GTP-binding; Lipoprotein; Myristate; Nucleotide-binding; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 181 AA 

Protein Sequence

Gene Ontology

 GO:0005794; C:Golgi apparatus; IDA:SGD.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IDA:SGD.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IDA:SGD.
 GO:0006893; P:Golgi to plasma membrane transport; IGI:SGD.
 GO:0006891; P:intra-Golgi vesicle-mediated transport; TAS:SGD.
 GO:0016236; P:macroautophagy; IMP:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR024156; Small_GTPase_ARF.
 IPR006689; Small_GTPase_ARF/SAR. 

Pfam

 PF00025; Arf 

SMART

 SM00177; ARF 

PROSITE

 PS51417; ARF 

PRINTS

 PR00328; SAR1GTPBP.