UniProt Accession

 FERM2_MOUSE; Q8CIB5; Q8C542; Q8K035 

Genbank Protein ID

 BC033436; BC034168; AK079588 

Genbank Nucleotide ID

 AAH33436.1; AAH34168.1; BAC37692.1 

Protein Name

 Fermitin family homolog 2 

Protein Synonyms/Alias

 Kindlin-2; Pleckstrin homology domain-containing family C member 1 

Gene Name

 Fermt2 

Gene Synonyms/Alias

 Plekhc1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
128	KAVSDICKTFNIRHP	ubiquitination	[1]
393	KLTLKGYKQYWCTFK	acetylation	[2]
400	KQYWCTFKDTSISCY	acetylation	[2]
555	QMSLIEAKMRFIQAW	ubiquitination	[3]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF4 and in Wnt signaling. 

Sequence Annotation

 DOMAIN 189 661 FERM.
 DOMAIN 380 476 PH.
 REGION 40 81 Interaction with membranes containing
 BINDING 383 383 Phosphatidylinositol phosphate (By
 MOD_RES 159 159 Phosphoserine.
 MOD_RES 181 181 Phosphoserine (By similarity).
 MOD_RES 339 339 Phosphoserine (By similarity).
 MOD_RES 351 351 Phosphoserine (By similarity).
 MOD_RES 666 666 Phosphoserine (By similarity).  

Keyword

 Cell adhesion; Cell junction; Cell membrane; Cell projection; Cell shape; Complete proteome; Cytoplasm; Cytoskeleton; Lipid-binding; Membrane; Nucleus; Phosphoprotein; Reference proteome; Wnt signaling pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 680 AA 

Protein Sequence

Gene Ontology

 GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
 GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0031234; C:extrinsic to internal side of plasma membrane; ISS:UniProtKB.
 GO:0005925; C:focal adhesion; ISS:UniProtKB.
 GO:0031674; C:I band; IEA:UniProtKB-SubCell.
 GO:0031258; C:lamellipodium membrane; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0005547; F:phosphatidylinositol-3,4,5-trisphosphate binding; ISS:UniProtKB.
 GO:0048041; P:focal adhesion assembly; IMP:UniProtKB.
 GO:0033622; P:integrin activation; IMP:UniProtKB.
 GO:0007229; P:integrin-mediated signaling pathway; ISS:UniProtKB.
 GO:0072657; P:protein localization to membrane; IMP:UniProtKB.
 GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
 GO:0034446; P:substrate adhesion-dependent cell spreading; IMP:UniProtKB.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; ISS:UniProtKB.
 GO:0016055; P:Wnt receptor signaling pathway; ISS:UniProtKB. 

Interpro

 IPR019749; Band_41_domain.
 IPR014352; FERM/acyl-CoA-bd_prot_3-hlx.
 IPR019748; FERM_central.
 IPR018979; FERM_N.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 

Pfam

 PF00373; FERM_M
 PF09379; FERM_N
 PF00169; PH 

SMART

 SM00295; B41
 SM00233; PH 

PROSITE

 PS00660; FERM_1
 PS00661; FERM_2
 PS50057; FERM_3
 PS50003; PH_DOMAIN 

PRINTS