UniProt Accession

 DX39B_MOUSE; Q9Z1N5; Q8HW97 

Genbank Protein ID

 AF118128; AY255786; AY255787; AC007080; AK088867; AK051034; BC011067; BC024859 

Genbank Nucleotide ID

 AAD13115.1; AAP91685.1; AAP91686.1; AAD30177.1; BAC40624.1; BAC34505.1; AAH11067.1; AAH24859.1 

Protein Name

 Spliceosome RNA helicase Ddx39b 

Protein Synonyms/Alias

 56 kDa U2AF65-associated protein; DEAD box protein UAP56; HLA-B-associated transcript 1 protein 

Gene Name

 Ddx39b 

Gene Synonyms/Alias

 Bat1; Bat1a; Uap56 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
36	APAKKDVKGSYVSIH	ubiquitination	[1]
53	GFRDFLLKPELLRAI	ubiquitination	[1]
131	ELAFQISKEYERFSK	ubiquitination	[1]
155	FFGGLSIKKDEEVLK	ubiquitination	[1]
222	FRMTPHEKQVMMFSA	acetylation	[2]
241	EIRPVCRKFMQDPME	acetylation	[2]
241	EIRPVCRKFMQDPME	ubiquitination	[1]
268	GLQQYYVKLKDNEKN	ubiquitination	[1]
334	LSRYQQFKDFQRRIL	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex (By similarity). 

Sequence Annotation

 DOMAIN 76 249 Helicase ATP-binding.
 DOMAIN 261 422 Helicase C-terminal.
 NP_BIND 89 96 ATP (By similarity).
 MOTIF 45 73 Q motif.
 MOTIF 196 199 DECD box.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 36 36 N6-acetyllysine (By similarity).
 MOD_RES 38 38 Phosphoserine (By similarity).
 MOD_RES 172 172 Phosphothreonine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Helicase; Hydrolase; mRNA processing; mRNA splicing; mRNA transport; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Spliceosome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 428 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
 GO:0000346; C:transcription export complex; IEA:Compara.
 GO:0005687; C:U4 snRNP; IEA:Compara.
 GO:0005688; C:U6 snRNP; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004004; F:ATP-dependent RNA helicase activity; IEA:Compara.
 GO:0030621; F:U4 snRNA binding; IEA:Compara.
 GO:0017070; F:U6 snRNA binding; IEA:Compara.
 GO:0046784; P:intronless viral mRNA export from host nucleus; IEA:Compara.
 GO:0010501; P:RNA secondary structure unwinding; IEA:Compara.
 GO:0000245; P:spliceosomal complex assembly; IEA:Compara. 

Interpro

 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR014014; RNA_helicase_DEAD_Q_motif. 

Pfam

 PF00270; DEAD
 PF00271; Helicase_C 

SMART

 SM00487; DEXDc
 SM00490; HELICc 

PROSITE

 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 

PRINTS