CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007899
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vacuolar transporter chaperone 4 
Protein Synonyms/Alias
 Phosphate metabolism protein 3 
Gene Name
 VTC4 
Gene Synonyms/Alias
 PHM3; YJL012C; J1345 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
64DKVYTFCKVKHSEVFacetylation[1]
75SEVFRRVKEVQEQVQubiquitination[2, 3]
129LNYTGFQKIIKKHDKacetylation[1]
137IIKKHDKKTGFILKPacetylation[1]
153FQVRLDSKPFFKENYacetylation[1]
190GDSSAGGKQQNFVRQubiquitination[3]
200NFVRQTTKYWVHPDNacetylation[1]
256LYYGRLRKDEGAEAHacetylation[1]
309RHVNDFLKGKYTVDQacetylation[1]
311VNDFLKGKYTVDQVFacetylation[1]
320TVDQVFAKMRKEGKKacetylation[1]
455HLVEPVPKFSKFIHGacetylation[1]
597DQISKIPKGTTFDTQubiquitination[3]
611QIRAPPGKTICVPVRubiquitination[3]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Component of the vacuolar transporter chaperone (VTC) complex, which plays a role in vacuolar membrane fusion. Required for SEC18/NSF activity in SNARE priming, membrane binding of LMA1 and V(0) trans-complex formation. 
Sequence Annotation
 DOMAIN 1 148 SPX.  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Membrane; Reference proteome; Transmembrane; Transmembrane helix; Vacuole. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 721 AA 
Protein Sequence
MKFGEHLSKS LIRQYSYYYI SYDDLKTELE DNLSKNNGQW TQELETDFLE SLEIELDKVY 60
TFCKVKHSEV FRRVKEVQEQ VQHTVRLLDS NNPPTQLDFE ILEEELSDII ADVHDLAKFS 120
RLNYTGFQKI IKKHDKKTGF ILKPVFQVRL DSKPFFKENY DELVVKISQL YDIARTSGRP 180
IKGDSSAGGK QQNFVRQTTK YWVHPDNITE LKLIILKHLP VLVFNTNKEF EREDSAITSI 240
YFDNENLDLY YGRLRKDEGA EAHRLRWYGG MSTDTIFVER KTHREDWTGE KSVKARFALK 300
ERHVNDFLKG KYTVDQVFAK MRKEGKKPMN EIENLEALAS EIQYVMLKKK LRPVVRSFYN 360
RTAFQLPGDA RVRISLDTEL TMVREDNFDG VDRTHKNWRR TDIGVDWPFK QLDDKDICRF 420
PYAVLEVKLQ TQLGQEPPEW VRELVGSHLV EPVPKFSKFI HGVATLLNDK VDSIPFWLPQ 480
MDVDIRKPPL PTNIEITRPG RSDNEDNDFD EDDEDDAALV AAMTNAPGNS LDIEESVGYG 540
ATSAPTSNTN HVVESANAAY YQRKIRNAEN PISKKYYEIV AFFDHYFNGD QISKIPKGTT 600
FDTQIRAPPG KTICVPVRVE PKVYFATERT YLSWLSISIL LGGVSTTLLT YGSPTAMIGS 660
IGFFITSLAV LIRTVMVYAK RVVNIRLKRA VDYEDKIGPG MVSVFLILSI LFSFFCNLVA 720
K 721 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0031310; C:intrinsic to vacuolar membrane; IDA:SGD.
 GO:0033254; C:vacuolar transporter chaperone complex; IPI:SGD.
 GO:0008976; F:polyphosphate kinase activity; IDA:SGD.
 GO:0016237; P:microautophagy; IDA:SGD.
 GO:0006797; P:polyphosphate metabolic process; IMP:SGD.
 GO:0007034; P:vacuolar transport; IMP:SGD.
 GO:0042144; P:vacuole fusion, non-autophagic; IMP:SGD. 
Interpro
 IPR003807; DUF202.
 IPR004331; SPX_N.
 IPR018966; VTC_domain. 
Pfam
 PF02656; DUF202
 PF03105; SPX
 PF09359; VTC 
SMART
  
PROSITE
 PS51382; SPX 
PRINTS