UniProt Accession

 TEST_HUMAN; Q9Y6M0; Q9NS34; Q9P2V6 

Genbank Protein ID

 AF058300; AB031329; AB031330; AB031331; AF058301; AF058301; AY359034; BC074999; BC075000 

Genbank Nucleotide ID

 AAD41588.1; BAA83520.1; BAA83521.1; BAA89532.1; AAF79019.1; AAF79020.1; AAQ89393.1; AAH74999.1; AAH75000.1 

Protein Name

 Testisin 

Protein Synonyms/Alias

 Eosinophil serine protease 1; ESP-1; Serine protease 21 

Gene Name

 PRSS21 

Gene Synonyms/Alias

 ESP1; TEST1; UNQ266/PRO303 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
142	PYDIALVKLSAPVTY	ubiquitination	[1]

Reference

 [1] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 

Functional Description

 Could regulate proteolytic events associated with testicular germ cell maturation. 

Sequence Annotation

 DOMAIN 42 286 Peptidase S1.
 ACT_SITE 82 82 Charge relay system (Potential).
 ACT_SITE 137 137 Charge relay system (Potential).
 ACT_SITE 238 238 Charge relay system (Potential).
 LIPID 288 288 GPI-anchor amidated serine (Potential).
 CARBOHYD 167 167 N-linked (GlcNAc...) (Potential).
 CARBOHYD 200 200 N-linked (GlcNAc...) (Potential).
 CARBOHYD 273 273 N-linked (GlcNAc...) (Potential).
 DISULFID 33 157 Potential.
 DISULFID 67 83 Potential.
 DISULFID 171 244 Potential.
 DISULFID 204 223 Potential.
 DISULFID 234 262 Potential.  

Keyword

 Alternative splicing; Cell membrane; Complete proteome; Disulfide bond; Glycoprotein; GPI-anchor; Hydrolase; Lipoprotein; Membrane; Polymorphism; Protease; Reference proteome; Serine protease; Signal; Zymogen. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 314 AA 

Protein Sequence

Gene Ontology

 GO:0031225; C:anchored to membrane; IEA:UniProtKB-KW.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005886; C:plasma membrane; TAS:ProtInc.
 GO:0004252; F:serine-type endopeptidase activity; IEA:Compara.
 GO:0008236; F:serine-type peptidase activity; TAS:ProtInc.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 

Interpro

 IPR001254; Peptidase_S1.
 IPR018114; Peptidase_S1_AS.
 IPR001314; Peptidase_S1A.
 IPR009003; Trypsin-like_Pept_dom. 

Pfam

 PF00089; Trypsin 

SMART

 SM00020; Tryp_SPc 

PROSITE

 PS50240; TRYPSIN_DOM
 PS00134; TRYPSIN_HIS
 PS00135; TRYPSIN_SER 

PRINTS

 PR00722; CHYMOTRYPSIN.