CPLM-023199

Genbank Nucleotide ID

A-kinase anchor protein 8-like

Protein Synonyms/Alias

AKAP8-like protein; Helicase A-binding protein 95; HAP95; Homologous to AKAP95 protein; HA95; Neighbor of A-kinase-anchoring protein 95; Neighbor of AKAP95

NAKAP; NAKAP95; HRIHFB2018

Homo sapiens (Human)

Position	Peptide	Type	References
257	FGFGNGMKQMRRTWK	acetylation	[1]
257	FGFGNGMKQMRRTWK	ubiquitination	[2, 3]
264	KQMRRTWKTWTTADF	ubiquitination	[2]
290	SPDEPDSKATRTDCS	ubiquitination	[4]
362	QDENGQTKRKLQAGK	ubiquitination	[4]
448	KKTEELRKTVEDLDG	ubiquitination	[2]
477	IAMEHFVKKVEAAHC	ubiquitination	[4]
533	ARSILNNKLISKKLE	acetylation	[5]
533	ARSILNNKLISKKLE	ubiquitination	[3, 6]
544	KKLERYLKGENPFTD	ubiquitination	[4]

[1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Could play a role in constitutive transport element (CTE)-mediated gene expression. Does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May regulate the initiation phase of DNA replication when associated with TMPO-beta.

ZN_FING 389 413 C2H2 AKAP95-type 1.
ZN_FING 482 507 C2H2 AKAP95-type 2.
MOTIF 274 279 Nuclear localization signal (Potential).
MOTIF 362 364 Nuclear localization signal (Potential).
MOD_RES 257 257 N6-acetyllysine.
MOD_RES 283 283 Phosphoserine.
MOD_RES 292 292 Phosphothreonine.
MOD_RES 297 297 Phosphoserine.

Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Zinc; Zinc-finger.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0016363; C:nuclear matrix; IDA:UniProtKB.
GO:0017151; F:DEAD/H-box RNA helicase binding; TAS:UniProtKB.
GO:0003677; F:DNA binding; IEA:InterPro.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.

IPR007071; AKAP95.
IPR015880; Znf_C2H2-like.