UniProt Accession

 NUP53_YEAST; Q03790; D6VZX4 

Genbank Protein ID

 Z49705; BK006946 

Genbank Nucleotide ID

 CAA89789.1; DAA10048.1 

Protein Name

 Nucleoporin NUP53 

Protein Synonyms/Alias

 Nuclear pore protein NUP53 

Gene Name

 NUP53 

Gene Synonyms/Alias

 YMR153W; YM8520.02 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
223	RILNFFDKNTKTTPN	acetylation	[1]
366	SEEIINNKTSSQTSL	ubiquitination	[2]
392	EGIFEKAKAKAVTSK	acetylation	[3]
394	IFEKAKAKAVTSKVR	acetylation	[3]
399	KAKAVTSKVRNAEFK	acetylation	[3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591] 

Functional Description

 Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP53 may play an important role in cell cycle regulation by inhibiting PSE1 transport functions during mitosis and sequestration of MAD1-MAD2 in a cell cycle-dependent manner. It also seems to play an important role in de novo NPC assembly by associating with nuclear membranes and driving their proliferation. 

Sequence Annotation

 REPEAT 124 125 FG 1.
 DOMAIN 247 352 RRM Nup35-type.
 REPEAT 264 265 FG 2.
 REPEAT 273 274 FG 3.
 REPEAT 470 471 FG 4.
 REGION 405 438 PSE1 binding.
 REGION 449 475 Required for nuclear membrane association
 MOD_RES 101 101 Phosphoserine.
 MOD_RES 297 297 Phosphoserine.
 MOD_RES 438 438 Phosphoserine.  

Keyword

 3D-structure; Cell cycle; Cell division; Complete proteome; Membrane; Mitosis; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; Translocation; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 475 AA 

Protein Sequence

Gene Ontology

 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0044613; C:nuclear pore central transport channel; IDA:SGD.
 GO:0044615; C:nuclear pore nuclear basket; IDA:SGD.
 GO:0005487; F:nucleocytoplasmic transporter activity; IPI:SGD.
 GO:0005543; F:phospholipid binding; IDA:SGD.
 GO:0003697; F:single-stranded DNA binding; IDA:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0007094; P:mitotic spindle assembly checkpoint; IMP:SGD.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0006607; P:NLS-bearing substrate import into nucleus; IMP:SGD.
 GO:0006999; P:nuclear pore organization; IGI:SGD.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:SGD.
 GO:0060188; P:regulation of protein desumoylation; IMP:SGD. 

Interpro

 IPR007846; RRM_NUP35_dom. 

Pfam

 PF05172; Nup35_RRM 

SMART

  

PROSITE

 PS51472; RRM_NUP35 

PRINTS