CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016542
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Neuronal calcium sensor 1 
Protein Synonyms/Alias
 NCS-1; Frequenin homolog 
Gene Name
 Ncs1 
Gene Synonyms/Alias
 Freq 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
50LDAAGFQKIYKQFFPubiquitination[1]
53AGFQKIYKQFFPFGDubiquitination[1]
106EKLRWAFKLYDLDNDubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N- type voltage-gated calcium channel (By similarity). 
Sequence Annotation
 DOMAIN 24 59 EF-hand 1.
 DOMAIN 60 95 EF-hand 2.
 DOMAIN 96 131 EF-hand 3.
 DOMAIN 144 179 EF-hand 4.
 REGION 174 190 Interaction with IL1RAPL1 (By
 LIPID 2 2 N-myristoyl glycine (By similarity).  
Keyword
 Calcium; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Golgi apparatus; Lipoprotein; Membrane; Metal-binding; Myristate; Postsynaptic cell membrane; Reference proteome; Repeat; Synapse. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 190 AA 
Protein Sequence
MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK IYKQFFPFGD 60
PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK LRWAFKLYDL DNDGYITRNE 120
MLDIVDAIYQ MVGNTVELPE EENTPEKRVD RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV 180
QALSLYDGLV 190 
Gene Ontology
 GO:0030424; C:axon; IDA:MGI.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0031410; C:cytoplasmic vesicle; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0030425; C:dendrite; IDA:MGI.
 GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0014069; C:postsynaptic density; IEA:UniProtKB-SubCell.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; TAS:MGI.
 GO:0005245; F:voltage-gated calcium channel activity; ISS:UniProtKB.
 GO:0048015; P:phosphatidylinositol-mediated signaling; IEA:Compara.
 GO:0045921; P:positive regulation of exocytosis; IEA:Compara.
 GO:0050806; P:positive regulation of synaptic transmission; TAS:MGI.
 GO:0010975; P:regulation of neuron projection development; ISS:UniProtKB. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR001125; Recoverin. 
Pfam
 PF00036; efhand 
SMART
 SM00054; EFh 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 
PRINTS
 PR00450; RECOVERIN.