CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023259
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat-containing protein 3 
Protein Synonyms/Alias
  
Gene Name
 WDR3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
57WDLRKGEKILILQGLubiquitination[1]
65ILILQGLKQEVTCLCacetylation[2]
65ILILQGLKQEVTCLCubiquitination[3]
238EPDPKKIKGSSPGIQubiquitination[4]
289VVNLAVDKTGRILACubiquitination[5, 6]
366IKTSAKIKSFDLIHSubiquitination[5]
474RQVVIGTKTGKLQLYubiquitination[4, 5, 6]
518FVTGGADKSVKFWDFubiquitination[5]
521GGADKSVKFWDFELVubiquitination[1, 5]
536KDENSTQKRLSVKQTubiquitination[3, 5, 7]
653HLFFTAGKDHKIKQWacetylation[6]
656FTAGKDHKIKQWDADubiquitination[5]
729EYEESVAKEDQPAVPubiquitination[3, 4]
748GDSYFTGKKTIETVKubiquitination[3, 4]
749DSYFTGKKTIETVKAubiquitination[5]
755KKTIETVKAAERIMEubiquitination[4]
906MAGLDYLKRECEAKSubiquitination[5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
 REPEAT 21 60 WD 1.
 REPEAT 63 102 WD 2.
 REPEAT 105 144 WD 3.
 REPEAT 147 186 WD 4.
 REPEAT 189 228 WD 5.
 REPEAT 277 316 WD 6.
 REPEAT 413 451 WD 7.
 REPEAT 453 493 WD 8.
 REPEAT 494 533 WD 9.
 REPEAT 547 586 WD 10.
 REPEAT 589 630 WD 11.
 REPEAT 631 670 WD 12.
 REPEAT 673 712 WD 13.
 MOD_RES 240 240 Phosphoserine.
 MOD_RES 241 241 Phosphoserine.  
Keyword
 Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 943 AA 
Protein Sequence
MGLTKQYLRY VASAVFGVIG SQKGNIVFVT LRGEKGRYVA VPACEHVFIW DLRKGEKILI 60
LQGLKQEVTC LCPSPDGLHL AVGYEDGSIR IFSLLSGEGN VTFNGHKAAI TTLKYDQLGG 120
RLASGSKDTD IIVWDVINES GLYRLKGHKD AITQALFLRE KNLLVTSGKD TMVKWWDLDT 180
QHCFKTMVGH RTEVWGLVLL SEEKRLITGA SDSELRVWDI AYLQEIEDPE EPDPKKIKGS 240
SPGIQDTLEA EDGAFETDEA PEDRILSCRK AGSIMREGRD RVVNLAVDKT GRILACHGTD 300
SVLELFCILS KKEIQKKMDK KMKKARKKAK LHSSKGEEED PEVNVEMSLQ DEIQRVTNIK 360
TSAKIKSFDL IHSPHGELKA VFLLQNNLVE LYSLNPSLPT PQPVRTSRIT IGGHRSDVRT 420
LSFSSDNIAV LSAAADSIKI WNRSTLQCIR TMTCEYALCS FFVPGDRQVV IGTKTGKLQL 480
YDLASGNLLE TIDAHDGALW SMSLSPDQRG FVTGGADKSV KFWDFELVKD ENSTQKRLSV 540
KQTRTLQLDE DVLCVSYSPN QKLLAVSLLD CTVKIFYVDT LKFFLSLYGH KLPVICMDIS 600
HDGALIATGS ADRNVKIWGL DFGDCHKSLF AHDDSVMYLQ FVPKSHLFFT AGKDHKIKQW 660
DADKFEHIQT LEGHHQEIWC LAVSPSGDYV VSSSHDKSLR LWERTREPLI LEEEREMERE 720
AEYEESVAKE DQPAVPGETQ GDSYFTGKKT IETVKAAERI MEAIELYREE TAKMKEHKAI 780
CKAAGKEVPL PSNPILMAYG SISPSAYVLE IFKGIKSSEL EESLLVLPFS YVPDILKLFN 840
EFIQLGSDVE LICRCLFFLL RIHFGQITSN QMLVPVIEKL RETTISKVSQ VRDVIGFNMA 900
GLDYLKRECE AKSEVMFFAD ATSHLEEKKR KRKKREKLIL TLT 943 
Gene Ontology
 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA. 
Interpro
 IPR020472; G-protein_beta_WD-40_rep.
 IPR007148; SSU_processome_Utp12.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF04003; Utp12
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.