UniProt Accession

 SYWC_MOUSE; P32921; Q80ZY4; Q99J58; Q9DC65 

Genbank Protein ID

 X69656; X69657; AK004541; BC003450; BC046232 

Genbank Nucleotide ID

 CAA49347.1; CAA49348.1; BAB23357.1; AAH03450.1; AAH46232.1 

Protein Name

 Tryptophan--tRNA ligase, cytoplasmic 

Protein Synonyms/Alias

 Tryptophanyl-tRNA synthetase; TrpRS; T1-TrpRS; T2-TrpRS 

Gene Name

 Wars 

Gene Synonyms/Alias

 Wrs 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
63	AAMGEEYKAGCPPGN	acetylation	[1]
106	AKGIDYDKLIVQFGS	ubiquitination	[2]
158	LDAYENKKPFYLYTG	acetylation	[3]
158	LDAYENKKPFYLYTG	succinylation	[3]
158	LDAYENKKPFYLYTG	ubiquitination	[2]
260	RNVVKIQKHVTFNQV	acetylation	[1]
370	IFLTDTAKQIKSKVN	ubiquitination	[2]
436	MLTGELKKTLIDVLQ	ubiquitination	[2]
454	AEHQARRKAVTEETV	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 T1-TrpRS has aminoacylation activity while T2-TrpRS lacks it. T1-TrpRS and T2-TrpRS possess angiostatic activity. T2- TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (By similarity). 

Sequence Annotation

 DOMAIN 12 68 WHEP-TRS.
 MOTIF 168 177 "HIGH" region.
 MOTIF 353 357 "KMSKS" region.  

Keyword

 Alternative splicing; Aminoacyl-tRNA synthetase; Angiogenesis; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 481 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004830; F:tryptophan-tRNA ligase activity; IEA:EC.
 GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
 GO:0045765; P:regulation of angiogenesis; IEA:Compara.
 GO:0006436; P:tryptophanyl-tRNA aminoacylation; IEA:InterPro. 

Interpro

 IPR001412; aa-tRNA-synth_I_CS.
 IPR002305; aa-tRNA-synth_Ic.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009068; S15_NS1_RNA-bd.
 IPR002306; Trp-tRNA-ligase.
 IPR000738; WHEP-TRS. 

Pfam

 PF00579; tRNA-synt_1b
 PF00458; WHEP-TRS 

SMART

 SM00991; WHEP-TRS 

PROSITE

 PS00178; AA_TRNA_LIGASE_I
 PS00762; WHEP_TRS_1
 PS51185; WHEP_TRS_2 

PRINTS

 PR01039; TRNASYNTHTRP.