CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019291
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Methylthioribulose-1-phosphate dehydratase 
Protein Synonyms/Alias
 MTRu-1-P dehydratase; APAF1-interacting protein; hAPIP 
Gene Name
 APIP 
Gene Synonyms/Alias
 CGI-29 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
21RRCGAQDKEHPRYLIacetylation[1]
65IAPSGVQKERIQPEDubiquitination[2, 3]
94SPSKKLKKSQCTPLFacetylation[4]
206VWGETWEKAKTMCECacetylation[5]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Catalyzes the dehydration of methylthioribulose-1- phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1- phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death. 
Sequence Annotation
 METAL 115 115 Zinc (Probable).
 METAL 117 117 Zinc (Probable).
 MOD_RES 87 87 Phosphoserine.  
Keyword
 Alternative splicing; Amino-acid biosynthesis; Apoptosis; Complete proteome; Cytoplasm; Lyase; Metal-binding; Methionine biosynthesis; Phosphoprotein; Polymorphism; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 242 AA 
Protein Sequence
MSGCDAREGD CCSRRCGAQD KEHPRYLIPE LCKQFYHLGW VTGTGGGISL KHGDEIYIAP 60
SGVQKERIQP EDMFVCDINE KDISGPSPSK KLKKSQCTPL FMNAYTMRGA GAVIHTHSKA 120
AVMATLLFPG REFKITHQEM IKGIKKCTSG GYYRYDDMLV VPIIENTPEE KDLKDRMAHA 180
MNEYPDSCAV LVRRHGVYVW GETWEKAKTM CECYDYLFDI AVSMKKVGLD PSQLPVGENG 240
IV 242 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0046570; F:methylthioribulose 1-phosphate dehydratase activity; TAS:Reactome.
 GO:0008270; F:zinc ion binding; IEA:HAMAP.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0019284; P:L-methionine biosynthetic process from S-adenosylmethionine; IEA:HAMAP.
 GO:0019509; P:L-methionine salvage from methylthioadenosine; IMP:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
 GO:0006595; P:polyamine metabolic process; TAS:Reactome.
 GO:0070372; P:regulation of ERK1 and ERK2 cascade; ISS:UniProtKB. 
Interpro
 IPR001303; Aldolase_II/adducin_N.
 IPR017714; MethylthioRu-1-P_deHdtase_MtnB.
 IPR027514; Salvage_MtnB_euk. 
Pfam
 PF00596; Aldolase_II 
SMART
 SM01007; Aldolase_II 
PROSITE
  
PRINTS