CPLM-019758

Genbank Nucleotide ID

Calcineurin B homologous protein 1

Protein Synonyms/Alias

Calcineurin B-like protein; Calcium-binding protein CHP; Calcium-binding protein p22; EF-hand calcium-binding domain-containing protein p22

Homo sapiens (Human)

Position	Peptide	Type	References
40	SRFTSLDKGENGTLS	ubiquitination	[1, 2, 3]
100	IEDNEKSKDVNGPEP	ubiquitination	[1, 3]
114	PLNSRSNKLHFAFRL	ubiquitination	[3]
126	FRLYDLDKDEKISRD	ubiquitination	[3]
178	ISFTEFVKVLEKVDV	ubiquitination	[1]
182	EFVKVLEKVDVEQKM	ubiquitination	[1, 3]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

Calcium-binding protein involved in different processes such as regulation of vesicular trafficking, plasma membrane Na(+)/H(+) exchanger and gene transcription. Involved in the constitutive exocytic membrane traffic. Mediates the association between microtubules and membrane-bound organelles of the endoplasmic reticulum and Golgi apparatus and is also required for the targeting and fusion of transcytotic vesicles (TCV) with the plasma membrane. Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Affects the pH sensitivity of SLC9A1/NHE1 by increasing its sensitivity at acidic pH. Required for the stabilization and localization of SLC9A1/NHE1 at the plasma membrane. Inhibits serum- and GTPase-stimulated Na(+)/H(+) exchange. Plays a role as an inhibitor of ribosomal RNA transcription by repressing the nucleolar UBF1 transcriptional activity. May sequester UBF1 in the nucleoplasm and limit its translocation to the nucleolus. Associates to the ribosomal gene promoter. Acts as a negative regulator of the calcineurin/NFAT signaling pathway. Inhibits NFAT nuclear translocation and transcriptional activity by suppressing the calcium-dependent calcineurin phosphatase activity. Also negatively regulates the kinase activity of the apoptosis-induced kinase STK17B. Inhibits both STK17B auto- and substrate- phosphorylations in a calcium-dependent manner.

DOMAIN 26 61 EF-hand 1.
DOMAIN 66 101 EF-hand 2.
DOMAIN 110 145 EF-hand 3.
DOMAIN 151 186 EF-hand 4.
REGION 143 185 Necessary for nuclear export signal.
MOTIF 2 6 Necessary for association with
MOTIF 138 147 Nuclear export signal 1 (By similarity).
MOTIF 176 185 Nuclear export signal 2 (By similarity).
LIPID 2 2 N-myristoyl glycine (By similarity).

3D-structure; Calcium; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Endoplasmic reticulum; Lipoprotein; Membrane; Metal-binding; Myristate; Nucleus; Phosphoprotein; Protein kinase inhibitor; Protein transport; Reference proteome; Repeat; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; IEA:Compara.
GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IEA:UniProtKB-SubCell.
GO:0000139; C:Golgi membrane; ISS:UniProtKB.
GO:0015630; C:microtubule cytoskeleton; ISS:UniProtKB.
GO:0005634; C:nucleus; ISS:UniProtKB.
GO:0005886; C:plasma membrane; IDA:UniProtKB.
GO:0030133; C:transport vesicle; ISS:UniProtKB.
GO:0005509; F:calcium ion binding; IDA:UniProtKB.
GO:0048306; F:calcium-dependent protein binding; IDA:UniProtKB.
GO:0019900; F:kinase binding; ISS:UniProtKB.
GO:0008017; F:microtubule binding; ISS:UniProtKB.
GO:0015459; F:potassium channel regulator activity; TAS:ProtInc.
GO:0004860; F:protein kinase inhibitor activity; IEA:UniProtKB-KW.
GO:0005215; F:transporter activity; ISS:UniProtKB.
GO:0017156; P:calcium ion-dependent exocytosis; IEA:Compara.
GO:0071468; P:cellular response to acidity; ISS:UniProtKB.
GO:0031122; P:cytoplasmic microtubule organization; ISS:UniProtKB.
GO:0022406; P:membrane docking; ISS:UniProtKB.
GO:0061025; P:membrane fusion; ISS:UniProtKB.
GO:0001578; P:microtubule bundle formation; ISS:UniProtKB.
GO:0070885; P:negative regulation of calcineurin-NFAT signaling cascade; IDA:UniProtKB.
GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IDA:UniProtKB.
GO:0010923; P:negative regulation of phosphatase activity; IDA:UniProtKB.
GO:0031953; P:negative regulation of protein autophosphorylation; ISS:UniProtKB.
GO:0042308; P:negative regulation of protein import into nucleus; IDA:UniProtKB.
GO:0006469; P:negative regulation of protein kinase activity; ISS:UniProtKB.
GO:0031397; P:negative regulation of protein ubiquitination; ISS:UniProtKB.
GO:0060050; P:positive regulation of protein glycosylation; ISS:UniProtKB.
GO:0090314; P:positive regulation of protein targeting to membrane; ISS:UniProtKB.
GO:0032417; P:positive regulation of sodium:hydrogen antiporter activity; IDA:UniProtKB.
GO:0006813; P:potassium ion transport; TAS:ProtInc.
GO:0006611; P:protein export from nucleus; ISS:UniProtKB.
GO:0051259; P:protein oligomerization; IEA:Compara.
GO:0050821; P:protein stabilization; ISS:UniProtKB.
GO:0051453; P:regulation of intracellular pH; IDA:UniProtKB.
GO:0007264; P:small GTPase mediated signal transduction; TAS:ProtInc.
GO:0045056; P:transcytosis; IEA:Compara.

IPR011992; EF-hand-like_dom.
IPR002048; EF_hand_dom.

PF13405; EF_hand_4
PF00036; efhand

PS00018; EF_HAND_1
PS50222; EF_HAND_2