CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006268
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 MHC class II transactivator 
Protein Synonyms/Alias
 CIITA 
Gene Name
 CIITA 
Gene Synonyms/Alias
 MHC2TA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
141MPAEVGQKSQKRPFPacetylation[1]
144EVGQKSQKRPFPEELacetylation[1, 2]
536LAGLFQKKLLRGCTLubiquitination[3]
Reference
 [1] Acetylation by PCAF enhances CIITA nuclear accumulation and transactivation of major histocompatibility complex class II genes.
 Spilianakis C, Papamatheakis J, Kretsovali A.
 Mol Cell Biol. 2000 Nov;20(22):8489-98. [PMID: 11046145]
 [2] The histone acetyltransferase domains of CREB-binding protein (CBP) and p300/CBP-associated factor are not necessary for cooperativity with the class II transactivator.
 Harton JA, Zika E, Ting JP.
 J Biol Chem. 2001 Oct 19;276(42):38715-20. [PMID: 11514574]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. 
Sequence Annotation
 DOMAIN 414 724 NACHT.
 REPEAT 985 1008 LRR 1.
 REPEAT 1016 1037 LRR 2.
 REPEAT 1045 1066 LRR 3.
 REPEAT 1073 1093 LRR 4.
 NP_BIND 420 427 ATP (Potential).  
Keyword
 Activator; ATP-binding; Complete proteome; Disease mutation; Leucine-rich repeat; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome; Repeat; SCID; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1130 AA 
Protein Sequence
MRCLAPRPAG SYLSEPQGSS QCATMELGPL EGGYLELLNS DADPLCLYHF YDQMDLAGEE 60
EIELYSEPDT DTINCDQFSR LLCDMEGDEE TREAYANIAE LDQYVFQDSQ LEGLSKDIFK 120
HIGPDEVIGE SMEMPAEVGQ KSQKRPFPEE LPADLKHWKP AEPPTVVTGS LLVRPVSDCS 180
TLPCLPLPAL FNQEPASGQM RLEKTDQIPM PFSSSSLSCL NLPEGPIQFV PTISTLPHGL 240
WQISEAGTGV SSIFIYHGEV PQASQVPPPS GFTVHGLPTS PDRPGSTSPF APSATDLPSM 300
PEPALTSRAN MTEHKTSPTQ CPAAGEVSNK LPKWPEPVEQ FYRSLQDTYG AEPAGPDGIL 360
VEVDLVQARL ERSSSKSLER ELATPDWAER QLAQGGLAEV LLAAKEHRRP RETRVIAVLG 420
KAGQGKSYWA GAVSRAWACG RLPQYDFVFS VPCHCLNRPG DAYGLQDLLF SLGPQPLVAA 480
DEVFSHILKR PDRVLLILDG FEELEAQDGF LHSTCGPAPA EPCSLRGLLA GLFQKKLLRG 540
CTLLLTARPR GRLVQSLSKA DALFELSGFS MEQAQAYVMR YFESSGMTEH QDRALTLLRD 600
RPLLLSHSHS PTLCRAVCQL SEALLELGED AKLPSTLTGL YVGLLGRAAL DSPPGALAEL 660
AKLAWELGRR HQSTLQEDQF PSADVRTWAM AKGLVQHPPR AAESELAFPS FLLQCFLGAL 720
WLALSGEIKD KELPQYLALT PRKKRPYDNW LEGVPRFLAG LIFQPPARCL GALLGPSAAA 780
SVDRKQKVLA RYLKRLQPGT LRARQLLELL HCAHEAEEAG IWQHVVQELP GRLSFLGTRL 840
TPPDAHVLGK ALEAAGQDFS LDLRSTGICP SGLGSLVGLS CVTRFRAALS DTVALWESLQ 900
QHGETKLLQA AEEKFTIEPF KAKSLKDVED LGKLVQTQRT RSSSEDTAGE LPAVRDLKKL 960
EFALGPVSGP QAFPKLVRIL TAFSSLQHLD LDALSENKIG DEGVSQLSAT FPQLKSLETL 1020
NLSQNNITDL GAYKLAEALP SLAASLLRLS LYNNCICDVG AESLARVLPD MVSLRVMDVQ 1080
YNKFTAAGAQ QLAASLRRCP HVETLAMWTP TIPFSVQEHL QQQDSRISLR 1130 
Gene Ontology
 GO:0016605; C:PML body; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0032403; F:protein complex binding; IDA:UniProtKB.
 GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:BHF-UCL.
 GO:0060333; P:interferon-gamma-mediated signaling pathway; TAS:Reactome.
 GO:0032966; P:negative regulation of collagen biosynthetic process; IC:BHF-UCL.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:BHF-UCL.
 GO:0045345; P:positive regulation of MHC class I biosynthetic process; IDA:BHF-UCL.
 GO:0045348; P:positive regulation of MHC class II biosynthetic process; IC:BHF-UCL.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:BHF-UCL.
 GO:0046677; P:response to antibiotic; IDA:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001611; Leu-rich_rpt.
 IPR025875; Leu-rich_rpt_4.
 IPR008095; MHC_II_transact.
 IPR007111; NACHT_NTPase. 
Pfam
 PF12799; LRR_4 
SMART
  
PROSITE
 PS51450; LRR
 PS50837; NACHT 
PRINTS
 PR01719; MHCIIACTVATR.