CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018390
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Valine--tRNA ligase 
Protein Synonyms/Alias
 Valyl-tRNA synthetase; ValRS 
Gene Name
 valS 
Gene Synonyms/Alias
 STM4475 
Created Date
 July 27, 2013 
Organism
 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 
NCBI Taxa ID
 99287 
Lysine Modification
Position
Peptide
Type
References
894DELARLAKEVAKIEGacetylation[1]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner (By similarity). 
Sequence Annotation
 MOTIF 42 52 "HIGH" region.
 MOTIF 554 558 "KMSKS" region.
 BINDING 557 557 ATP (By similarity).  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 951 AA 
Protein Sequence
MEKTYNPQDI EQPLYEHWEK QGYFKPNGDE SKESFCIMIP PPNVTGSLHM GHAFQQTIMD 60
TMIRYQRMQG KNTLWQVGTD HAGIATQMVV ERKIAAEEGK TRHDYGRDAF IDKIWQWKAE 120
SGGTITRQMR RLGNSVDWER ERFTMDEGLS NAVKEVFVRL YKEDLIYRGK RLVNWDPKLR 180
TAISDLEVEN RESKGSMWHI RYPLADGAKT ADGKDYLVVA TTRPETILGD TGVAVNPEDP 240
RYQSLIGKFV ILPLVNRRIP IVGDEHADME KGTGCVKITP AHDFNDYEVG KRHALPMINI 300
LTFDGDIRES AEVFDTKGEE SDVYSSEIPA EFQKLERFAA RKAVVAAVDA LGLLEEIKPH 360
DLTVPYGDRG GVVIEPMLTD QWYVRADVLA KPAVEAVENG DIQFVPKQYE NMYFSWMRDI 420
QDWCISRQLW WGHRIPAWYD NDGNVYVGRT EDEVRQENNL GADVALRQDE DVLDTWFSSA 480
LWTFSTLGWP ENTDALRQFH PTSVMVSGFD IIFFWIARMI MMTMHFIKDE NGKPQVPFHT 540
VYMTGLIRDD EGQKMSKSKG NVIDPLDMVD GISLPELLEK RTGNMMQPQM AEKIRKRTEK 600
QFPNGIEPHG TDALRFTLAA LASTGRDINW DMKRLEGYRN FCNKLWNASR FVLMNTEEQD 660
CGFNGGEMTL SLADRWILAE FNQTVKAYRD ALDSFRFDIA AGILYEFTWN QFCDWYLELT 720
KPVMTGGSES ELRGTRHTLV TVLEGLLRLA HPIIPFITET IWQRVKVICG ITADTIMLQP 780
FPEYNAAQVD EAALADTEWL KQAIVAVRNI RAEMNIAPGK PLELLLRGCS EEAVRRVNDN 840
RSFLQTLARL ESITVLPADD KGPVSVTKII DGAELLIPMA GLINKDDELA RLAKEVAKIE 900
GEIARIEGKL SNEGFVARAP EAVIAKEREK LDGYAEAKAK LIEQQAVISA L 951 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0004832; F:valine-tRNA ligase activity; IEA:HAMAP.
 GO:0006450; P:regulation of translational fidelity; IEA:GOC.
 GO:0006438; P:valyl-tRNA aminoacylation; IEA:HAMAP. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR002300; aa-tRNA-synth_Ia.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR010978; tRNA-bd_arm.
 IPR009080; tRNAsynth_1a_anticodon-bd.
 IPR013155; V/L/I-tRNA-synth_anticodon-bd.
 IPR019499; Val-tRNA_synth_tRNA-bd.
 IPR009008; Val/Leu/Ile-tRNA-synth_edit.
 IPR002303; Valyl-tRNA_ligase. 
Pfam
 PF08264; Anticodon_1
 PF00133; tRNA-synt_1
 PF10458; Val_tRNA-synt_C 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR00986; TRNASYNTHVAL.