UniProt Accession

 VPS4B_MOUSE; P46467; Q91W22; Q9R1C9 

Genbank Protein ID

 U10119; AF134119; BC003799 

Genbank Nucleotide ID

 AAA50497.1; AAD47570.1; AAH03799.1 

Protein Name

 Vacuolar protein sorting-associated protein 4B 

Protein Synonyms/Alias

 Suppressor of K(+) transport growth defect 1; Protein SKD1 

Gene Name

 Vps4b 

Gene Synonyms/Alias

 Skd1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
17	KAIDLASKAAQEDKA	ubiquitination	[1]
147	EGAKEALKEAVILPI	ubiquitination	[1]
163	FPHLFTGKRTPWRGI	ubiquitination	[1]
217	GESEKLVKNLFQLAR	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (By similarity). 

Sequence Annotation

 DOMAIN 4 82 MIT.
 NP_BIND 174 181 ATP (Potential).
 MOD_RES 102 102 Phosphoserine.
 MOD_RES 108 108 Phosphoserine (By similarity).  

Keyword

 3D-structure; ATP-binding; Cell cycle; Cell division; Coiled coil; Complete proteome; Endosome; Hydrolase; Membrane; Nucleotide-binding; Phosphoprotein; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 444 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:MGI.
 GO:0005769; C:early endosome; IEA:Compara.
 GO:0005768; C:endosome; ISO:MGI.
 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005764; C:lysosome; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005774; C:vacuolar membrane; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; ISO:MGI.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0030301; P:cholesterol transport; IMP:MGI.
 GO:0016197; P:endosomal transport; ISO:MGI.
 GO:0007032; P:endosome organization; IMP:MGI.
 GO:0032510; P:endosome to lysosome transport via multivesicular body sorting pathway; IEA:Compara.
 GO:0032367; P:intracellular cholesterol transport; IEA:Compara.
 GO:0006813; P:potassium ion transport; IDA:MGI.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0033993; P:response to lipid; IEA:Compara. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR007330; MIT.
 IPR027417; P-loop_NTPase.
 IPR015415; Vps4_C. 

Pfam

 PF00004; AAA
 PF04212; MIT
 PF09336; Vps4_C 

SMART

 SM00382; AAA
 SM00745; MIT 

PROSITE

 PS00674; AAA 

PRINTS