CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007940
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Aldehyde dehydrogenase, mitochondrial 
Protein Synonyms/Alias
 AHD-M1; ALDH class 2; ALDH-E2; ALDHI 
Gene Name
 Aldh2 
Gene Synonyms/Alias
 Ahd-1; Ahd1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
54WHDAVSRKTFPTVNPacetylation[1, 2, 3]
54WHDAVSRKTFPTVNPubiquitination[4]
75CQVAEGNKEDVDKAVacetylation[2, 3, 5, 6, 7]
80GNKEDVDKAVKAARAacetylation[2, 3, 5, 7]
83EDVDKAVKAARAAFQacetylation[2, 7]
157YYAGWADKYHGKTIPacetylation[2]
157YYAGWADKYHGKTIPubiquitination[4]
161WADKYHGKTIPIDGDacetylation[2]
161WADKYHGKTIPIDGDubiquitination[4]
282AAGSSNLKRVTLELGacetylation[3]
282AAGSSNLKRVTLELGubiquitination[4]
370QVDETQFKKILGYIKacetylation[1, 2, 3, 5, 6, 7, 8, 9, 10]
370QVDETQFKKILGYIKubiquitination[4]
371VDETQFKKILGYIKSacetylation[1]
377KKILGYIKSGQQEGAacetylation[1, 2, 3, 5, 7]
377KKILGYIKSGQQEGAubiquitination[4]
385SGQQEGAKLLCGGGAacetylation[2, 3, 5, 6, 7, 11]
385SGQQEGAKLLCGGGAubiquitination[4]
409PTVFGDVKDGMTIAKacetylation[2, 3, 5, 7]
409PTVFGDVKDGMTIAKubiquitination[4]
416KDGMTIAKEEIFGPVacetylation[5]
428GPVMQILKFKTIEEVacetylation[1, 2, 3, 5, 7, 9]
430VMQILKFKTIEEVVGacetylation[1, 2, 3, 7, 9, 10]
430VMQILKFKTIEEVVGubiquitination[4]
443VGRANDSKYGLAAAVacetylation[1, 2, 3, 5, 7, 12, 13]
443VGRANDSKYGLAAAVubiquitination[4]
453LAAAVFTKDLDKANYacetylation[2, 3, 7, 9]
453LAAAVFTKDLDKANYubiquitination[4]
513EVKTVTVKVPQKNS*acetylation[7]
513EVKTVTVKVPQKNS*ubiquitination[4]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [6] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [7] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [8] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [9] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [10] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [11] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [12] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [13] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405
Functional Description
 Is capable of converting retinaldehyde to retinoic acid. 
Sequence Annotation
 NP_BIND 264 269 NAD (By similarity).
 ACT_SITE 287 287 Proton acceptor (By similarity).
 ACT_SITE 321 321 Nucleophile (By similarity).
 MOD_RES 20 20 N-acetylserine (Probable).
 MOD_RES 370 370 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Direct protein sequencing; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 519 AA 
Protein Sequence
MLRAALTTVR RGPRLSRLLS AAATSAVPAP NHQPEVFCNQ IFINNEWHDA VSRKTFPTVN 60
PSTGEVICQV AEGNKEDVDK AVKAARAAFQ LGSPWRRMDA SDRGRLLYRL ADLIERDRTY 120
LAALETLDNG KPYVISYLVD LDMVLKCLRY YAGWADKYHG KTIPIDGDFF SYTRHEPVGV 180
CGQIIPWNFP LLMQAWKLGP ALATGNVVVM KVAEQTPLTA LYVANLIKEA GFPPGVVNIV 240
PGFGPTAGAA IASHEGVDKV AFTGSTEVGH LIQVAAGSSN LKRVTLELGG KSPNIIMSDA 300
DMDWAVEQAH FALFFNQGQC CCAGSRTFVQ ENVYDEFVER SVARAKSRVV GNPFDSRTEQ 360
GPQVDETQFK KILGYIKSGQ QEGAKLLCGG GAAADRGYFI QPTVFGDVKD GMTIAKEEIF 420
GPVMQILKFK TIEEVVGRAN DSKYGLAAAV FTKDLDKANY LSQALQAGTV WINCYDVFGA 480
QSPFGGYKMS GSGRELGEYG LQAYTEVKTV TVKVPQKNS 519 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004029; F:aldehyde dehydrogenase (NAD) activity; IEA:EC.
 GO:0006068; P:ethanol catabolic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR016161; Ald_DH/histidinol_DH.
 IPR016163; Ald_DH_C.
 IPR016160; Ald_DH_CS.
 IPR016162; Ald_DH_N.
 IPR015590; Aldehyde_DH_dom. 
Pfam
 PF00171; Aldedh 
SMART
  
PROSITE
 PS00070; ALDEHYDE_DEHYDR_CYS
 PS00687; ALDEHYDE_DEHYDR_GLU 
PRINTS