UniProt Accession

 EX3_ECOLI; P09030 

Genbank Protein ID

 X13002; M22592; U00096; AP009048 

Genbank Nucleotide ID

 CAA31424.1; AAA24767.1; AAC74819.1; BAA15540.1 

Protein Name

 Exodeoxyribonuclease III 

Protein Synonyms/Alias

 EXO III; Exonuclease III; AP endonuclease VI 

Gene Name

 xthA 

Gene Synonyms/Alias

 xth; b1749; JW1738 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
24	QLEAIVEKHQPDVIG	acetylation	[1]
36	VIGLQETKVHDDMFP	acetylation	[1]
49	FPLEEVAKLGYNVFY	acetylation	[1]
70	YGVALLTKETPIAVR	acetylation	[1]
121	ESRDHPIKFPAKAQF	acetylation	[1]
141	NYLETELKRDNPVLI	acetylation	[1]
141	NYLETELKRDNPVLI	pupylation	[2]
176	KRWLRTGKCSFLPEE	acetylation	[1]
255	YEIRSMEKPSDHAPV	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222] 

Functional Description

 Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase and ribonuclease H activities. 

Sequence Annotation

 ACT_SITE 109 109 By similarity.
 ACT_SITE 151 151 Proton donor/acceptor (By similarity).
 METAL 34 34 Magnesium 1 (By similarity).
 METAL 151 151 Magnesium 2 (By similarity).
 METAL 153 153 Magnesium 2 (By similarity).
 METAL 258 258 Magnesium 1 (By similarity).  

Keyword

 3D-structure; Complete proteome; DNA damage; DNA repair; Exonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 268 AA 

Protein Sequence

Gene Ontology

 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0004519; F:endonuclease activity; IEA:InterPro.
 GO:0008853; F:exodeoxyribonuclease III activity; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0006974; P:response to DNA damage stimulus; IDA:EcoCyc. 

Interpro

 IPR020847; AP_endonuclease_F1_BS.
 IPR020848; AP_endonuclease_F1_CS.
 IPR005135; Endo/exonuclease/phosphatase.
 IPR004808; ExoDNase_III. 

Pfam

 PF03372; Exo_endo_phos 

SMART

  

PROSITE

 PS00726; AP_NUCLEASE_F1_1
 PS00727; AP_NUCLEASE_F1_2
 PS00728; AP_NUCLEASE_F1_3
 PS51435; AP_NUCLEASE_F1_4 

PRINTS