CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019773
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mothers against decapentaplegic homolog 5 
Protein Synonyms/Alias
 MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5 
Gene Name
 SMAD5 
Gene Synonyms/Alias
 MADH5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
64SSPGQPSKCVTIPRSubiquitination[1, 2]
82RLQVSHRKGLPHVIYubiquitination[2]
306FTDPSNNKSRFCLGLubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). 
Sequence Annotation
 DOMAIN 13 137 MH1.
 DOMAIN 271 465 MH2.
 METAL 65 65 Zinc (By similarity).
 METAL 110 110 Zinc (By similarity).
 METAL 122 122 Zinc (By similarity).
 METAL 127 127 Zinc (By similarity).
 MOD_RES 2 2 N-acetylthreonine.
 MOD_RES 463 463 Phosphoserine.
 MOD_RES 465 465 Phosphoserine.  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 465 AA 
Protein Sequence
MTSMASLFSF TSPAVKRLLG WKQGDEEEKW AEKAVDALVK KLKKKKGAME ELEKALSSPG 60
QPSKCVTIPR SLDGRLQVSH RKGLPHVIYC RVWRWPDLQS HHELKPLDIC EFPFGSKQKE 120
VCINPYHYKR VESPVLPPVL VPRHNEFNPQ HSLLVQFRNL SHNEPHMPQN ATFPDSFHQP 180
NNTPFPLSPN SPYPPSPASS TYPNSPASSG PGSPFQLPAD TPPPAYMPPD DQMGQDNSQP 240
MDTSNNMIPQ IMPSISSRDV QPVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT 300
DPSNNKSRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD SSIFVQSRNC 360
NFHHGFHPTT VCKIPSSCSL KIFNNQEFAQ LLAQSVNHGF EAVYELTKMC TIRMSFVKGW 420
GAEYHRQDVT STPCWIEIHL HGPLQWLDKV LTQMGSPLNP ISSVS 465 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005667; C:transcription factor complex; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0000979; F:RNA polymerase II core promoter sequence-specific DNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0030618; F:transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; TAS:BHF-UCL.
 GO:0030509; P:BMP signaling pathway; ISS:UniProtKB.
 GO:0060348; P:bone development; IEA:Compara.
 GO:0060048; P:cardiac muscle contraction; IEA:Compara.
 GO:0051216; P:cartilage development; IEA:Compara.
 GO:0071773; P:cellular response to BMP stimulus; ISS:BHF-UCL.
 GO:0071407; P:cellular response to organic cyclic compound; IEA:Compara.
 GO:0009880; P:embryonic pattern specification; ISS:UniProtKB.
 GO:0030218; P:erythrocyte differentiation; IEA:Compara.
 GO:0007281; P:germ cell development; IEA:Compara.
 GO:0001880; P:Mullerian duct regression; IEA:Compara.
 GO:0002051; P:osteoblast fate commitment; IEA:Compara.
 GO:0045669; P:positive regulation of osteoblast differentiation; IEA:Compara.
 GO:1901522; P:positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus; ISS:BHF-UCL.
 GO:0006468; P:protein phosphorylation; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; NAS:UniProtKB.
 GO:0001657; P:ureteric bud development; IEA:Compara. 
Interpro
 IPR013790; Dwarfin.
 IPR003619; MAD_homology1_Dwarfin-type.
 IPR013019; MAD_homology_MH1.
 IPR017855; SMAD_dom-like.
 IPR001132; SMAD_dom_Dwarfin-type.
 IPR008984; SMAD_FHA_domain. 
Pfam
 PF03165; MH1
 PF03166; MH2 
SMART
 SM00523; DWA
 SM00524; DWB 
PROSITE
 PS51075; MH1
 PS51076; MH2 
PRINTS