UniProt Accession

 I3L0J9_HUMAN; I3L0J9 

Genbank Protein ID

 AC130343 

Genbank Nucleotide ID

  

Protein Name

 Pre-mRNA-processing-splicing factor 8 

Protein Synonyms/Alias

  

Gene Name

 PRPF8 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
29	PDYMSEEKLQEKARK	ubiquitination	[1, 2, 3]
33	SEEKLQEKARKWQQL	ubiquitination	[3]
36	KLQEKARKWQQLQAK	ubiquitination	[4]
43	KWQQLQAKRYAEKRK	ubiquitination	[4]
68	MPPEHVRKIIRDHGD	ubiquitination	[4]
163	QPLRDSRKYVNGSTY	ubiquitination	[1, 3, 4, 5]
212	LKAFFTSKALNMAIP	ubiquitination	[1, 2, 3, 4, 6, 7]
223	MAIPGGPKFEPLVRD	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
246	NEFNDINKIIIRQPI	ubiquitination	[1, 2, 7]
311	ISHRHSVKSQEPLPD	ubiquitination	[1, 2, 7]
373	ALDIPLVKNWYREHC	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
387	CPAGQPVKVRVSYQK	ubiquitination	[3, 4, 7]
394	KVRVSYQKLLKYYVL	ubiquitination	[1, 3, 4, 6]
397	VSYQKLLKYYVLNAL	ubiquitination	[4, 6, 7]
405	YYVLNALKHRPPKAQ	ubiquitination	[4, 6, 7]
425	FRSFKATKFFQSTKL	ubiquitination	[4, 6]
431	TKFFQSTKLDWVEVG	ubiquitination	[7]
456	LNLLIHRKNLNYLHL	ubiquitination	[4]
470	LDYNFNLKPVKTLTT	ubiquitination	[3, 6]
473	NFNLKPVKTLTTKER	ubiquitination	[3, 4]
478	PVKTLTTKERKKSRF	ubiquitination	[4]
500	REVLRLTKLVVDSHV	ubiquitination	[1, 3, 4]
554	IRMCKDLKHLIYYRF	ubiquitination	[1, 4, 6, 7]
568	FNTGPVGKGPGCGFW	ubiquitination	[7]
615	RHSKGVAKTVTKQRV	ubiquitination	[1]
619	GVAKTVTKQRVESHF	ubiquitination	[1]
647	DMMPEGIKQNKARTI	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
650	PEGIKQNKARTILQH	ubiquitination	[3, 4, 6]
666	SEAWRCWKANIPWKV	ubiquitination	[4]
672	WKANIPWKVPGLPTP	ubiquitination	[2, 3, 4, 6]
691	ILRYVKAKADWWTNT	ubiquitination	[3, 4, 6, 7]
714	RRGATVDKTVCKKNL	ubiquitination	[3]
730	RLTRLYLKAEQERQH	ubiquitination	[1, 3, 4, 5, 6, 7]
778	PFPPLSYKHDTKLLI	ubiquitination	[3, 4, 6]
782	LSYKHDTKLLILALE	ubiquitination	[4, 7]
792	ILALERLKEAYSVKS	ubiquitination	[4]
798	LKEAYSVKSRLNQSQ	ubiquitination	[3, 4]
877	YLWYEADKRRLFPPW	ubiquitination	[1, 2, 6, 7]
886	RLFPPWIKPADTEPP	ubiquitination	[1, 4, 7]
928	MLESRFEKMYEKIDL	ubiquitination	[1, 4]
932	RFEKMYEKIDLTLLN	ubiquitination	[1, 3, 4]
957	IADYMTAKNNVVINY	ubiquitination	[1, 4, 7]
965	NNVVINYKDMNHTNS	ubiquitination	[1, 3, 4, 6, 7]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1013 AA 

Protein Sequence

Gene Ontology

 GO:0005681; C:spliceosomal complex; IEA:InterPro.
 GO:0000398; P:mRNA splicing, via spliceosome; IEA:InterPro. 

Interpro

 IPR012591; Pre-mRNA-splicing_factor-8.
 IPR012592; PROCN.
 IPR019582; RRM_spliceosomal_PrP8. 

Pfam

 PF08082; PRO8NT
 PF08083; PROCN
 PF10598; RRM_4 

SMART

  

PROSITE

  

PRINTS