UniProt Accession

 GREA_ECOLI; P0A6W5; P21346; P78111; Q2M931; Q8X9K9 

Genbank Protein ID

 X54718; U01376; U18997; U00096; AP009048 

Genbank Nucleotide ID

 CAA38521.1; AAA97506.1; AAA57982.1; AAC76213.2; BAE77225.1 

Protein Name

 Transcription elongation factor GreA 

Protein Synonyms/Alias

 Transcript cleavage factor GreA 

Gene Name

 greA 

Gene Synonyms/Alias

 b3181; JW3148 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
13	MTLRGAEKLREELDF	acetylation	[1]
22	REELDFLKSVRRPEI	acetylation	[1]
43	AREHGDLKENAEYHA	acetylation	[1]
63	GFCEGRIKDIEAKLS	acetylation	[1]
68	RIKDIEAKLSNAQVI	acetylation	[1]
79	AQVIDVTKMPNNGRV	acetylation	[1]
116	GDDEADFKQNLISVN	acetylation	[1]
133	IARGLIGKEEDDVVV	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides. 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; DNA-binding; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 158 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0001108; F:bacterial-type RNA polymerase holo enzyme binding; IDA:EcoCyc.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0006354; P:DNA-dependent transcription, elongation; IDA:EcoCyc.
 GO:0032784; P:regulation of DNA-dependent transcription, elongation; IDA:EcoCyc. 

Interpro

 IPR018151; TF_GreA/GreB_CS.
 IPR006359; Tscrpt_elong_fac_GreA.
 IPR023459; Tscrpt_elong_fac_GreA/B.
 IPR001437; Tscrpt_elong_fac_GreA/B_C.
 IPR022691; Tscrpt_elong_fac_GreA/B_N. 

Pfam

 PF01272; GreA_GreB
 PF03449; GreA_GreB_N 

SMART

  

PROSITE

 PS00829; GREAB_1
 PS00830; GREAB_2 

PRINTS