UniProt Accession

 IDH2_YEAST; P28241; D6W2J3 

Genbank Protein ID

 M74131; X94335; Z75043; X90518; BK006948 

Genbank Nucleotide ID

 AAA34702.1; CAA64054.1; CAA99335.1; CAA62110.1; DAA10909.1 

Protein Name

 Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial 

Protein Synonyms/Alias

 Isocitric dehydrogenase; NAD(+)-specific ICDH 

Gene Name

 IDH2 

Gene Synonyms/Alias

 YOR136W; O3326; YOR3326W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
36	KPNPSTGKYTVSFIE	acetylation	[1]
53	GIGPEISKSVKKIFS	acetylation	[1]
100	TKNLVALKGPLATPI	acetylation	[1]
220	GLFVNVAKELSKEYP	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Performs an essential role in the oxidative function of the citric acid cycle. Also binds RNA; specifically to the 5'- untranslated leaders of mitochondrial mRNAs. 

Sequence Annotation

 METAL 237 237 Magnesium or manganese (By similarity).
 METAL 263 263 Magnesium or manganese (By similarity).
 METAL 267 267 Magnesium or manganese (By similarity).
 BINDING 119 119 Substrate (By similarity).
 BINDING 129 129 Substrate (By similarity).
 BINDING 150 150 Substrate (By similarity).
 BINDING 237 237 Substrate (By similarity).
 MOD_RES 105 105 Phosphothreonine.
 MOD_RES 153 153 Phosphothreonine.
 MOD_RES 327 327 Phosphothreonine.
 MOD_RES 349 349 Phosphothreonine.  

Keyword

 3D-structure; Allosteric enzyme; Complete proteome; Direct protein sequencing; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; RNA-binding; Transit peptide; Tricarboxylic acid cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 369 AA 

Protein Sequence

Gene Ontology

 GO:0005962; C:mitochondrial isocitrate dehydrogenase complex (NAD+); IDA:SGD.
 GO:0004449; F:isocitrate dehydrogenase (NAD+) activity; IEA:EC.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006537; P:glutamate biosynthetic process; TAS:SGD.
 GO:0006102; P:isocitrate metabolic process; TAS:SGD.
 GO:0006099; P:tricarboxylic acid cycle; TAS:SGD. 

Interpro

 IPR019818; IsoCit/isopropylmalate_DH_CS.
 IPR001804; Isocitrate/isopropylmalate_DH.
 IPR004434; Isocitrate_DH_NAD.
 IPR024084; IsoPropMal-DH-like_dom. 

Pfam

 PF00180; Iso_dh 

SMART

  

PROSITE

 PS00470; IDH_IMDH 

PRINTS