UniProt Accession

 ETFA_MOUSE; Q99LC5; Q3THD7; Q3V000; Q4V9X5; Q8BMD3; Q8BMU7 

Genbank Protein ID

 AK028118; AK032830; AK133525; AK167572; AK168321; BC003432; BC096645 

Genbank Nucleotide ID

 BAC25758.1; BAC28046.1; BAE21705.1; BAE39635.1; BAE40260.1; AAH03432.1; AAH96645.1 

Protein Name

 Electron transfer flavoprotein subunit alpha, mitochondrial 

Protein Synonyms/Alias

 Alpha-ETF 

Gene Name

 Etfa 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
59	SCLVAGTKCDKVVQD	acetylation	[1, 2, 3, 4, 5]
59	SCLVAGTKCDKVVQD	succinylation	[4]
62	VAGTKCDKVVQDLCK	acetylation	[1, 2, 3, 5]
69	KVVQDLCKVAGVAKV	acetylation	[1, 2, 3, 4, 5, 6, 7, 8]
69	KVVQDLCKVAGVAKV	succinylation	[4]
69	KVVQDLCKVAGVAKV	ubiquitination	[9]
75	CKVAGVAKVLVAQHD	acetylation	[1, 2, 3, 4, 5, 6]
75	CKVAGVAKVLVAQHD	succinylation	[4]
85	VAQHDAYKGLLPEEL	acetylation	[1, 2, 3, 4, 5, 6, 10]
85	VAQHDAYKGLLPEEL	succinylation	[4]
101	PLILETQKQFSYTHI	acetylation	[3]
117	AGASAFGKNLLPRVA	acetylation	[5]
126	LLPRVAAKLNVAPVS	acetylation	[1, 3]
126	LLPRVAAKLNVAPVS	ubiquitination	[9]
139	VSDIIEIKSPDTFVR	acetylation	[1, 2, 3, 5, 10]
139	VSDIIEIKSPDTFVR	ubiquitination	[9]
158	GNALCTVKCDEKVKV	acetylation	[1, 2, 3, 4, 5, 8]
158	GNALCTVKCDEKVKV	succinylation	[4]
162	CTVKCDEKVKVFSVR	acetylation	[2, 5]
164	VKCDEKVKVFSVRGT	acetylation	[2, 3, 5, 6]
164	VKCDEKVKVFSVRGT	ubiquitination	[9]
187	GGSASSEKAPSSSSV	acetylation	[4]
187	GGSASSEKAPSSSSV	succinylation	[4]
203	ISEWLDQKLTKSDRP	acetylation	[1, 3, 4]
203	ISEWLDQKLTKSDRP	succinylation	[4]
203	ISEWLDQKLTKSDRP	ubiquitination	[9]
206	WLDQKLTKSDRPELT	acetylation	[2, 4, 5]
206	WLDQKLTKSDRPELT	succinylation	[4]
216	RPELTGAKVVVSGGR	acetylation	[2, 3, 4, 5]
216	RPELTGAKVVVSGGR	succinylation	[4]
216	RPELTGAKVVVSGGR	ubiquitination	[9]
226	VSGGRGLKSGENFKL	acetylation	[2, 3, 4, 5]
226	VSGGRGLKSGENFKL	succinylation	[4]
226	VSGGRGLKSGENFKL	ubiquitination	[9]
232	LKSGENFKLLYDLAD	acetylation	[1, 2, 3, 4, 5, 10, 11]
232	LKSGENFKLLYDLAD	succinylation	[4]
294	LAGMKDSKTIVAINK	ubiquitination	[9]
301	KTIVAINKDPEAPIF	acetylation	[1, 4, 6]
301	KTIVAINKDPEAPIF	succinylation	[4]
331	PEMTEILKKK*****	acetylation	[2, 3, 4, 5, 10]
331	PEMTEILKKK*****	succinylation	[4]
331	PEMTEILKKK*****	ubiquitination	[9]
332	EMTEILKKK******	acetylation	[2]

Reference

 [1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [6] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [8] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [9] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [10] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [11] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379] 

Functional Description

 The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (By similarity). 

Sequence Annotation

 NP_BIND 274 302 FAD (Potential).  

Keyword

 Complete proteome; Direct protein sequencing; Electron transport; FAD; Flavoprotein; Mitochondrion; Reference proteome; Transit peptide; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 333 AA 

Protein Sequence

Gene Ontology

 GO:0017133; C:mitochondrial electron transfer flavoprotein complex; TAS:MGI.
 GO:0009055; F:electron carrier activity; TAS:MGI.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:Compara.
 GO:0016491; F:oxidoreductase activity; IEA:Compara.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW. 

Interpro

 IPR001308; ETF_a.
 IPR014730; ETF_a/b_N.
 IPR014731; ETF_asu_C.
 IPR018206; ETF_asu_C_CS.
 IPR014729; Rossmann-like_a/b/a_fold. 

Pfam

 PF01012; ETF
 PF00766; ETF_alpha 

SMART

 SM00893; ETF 

PROSITE

 PS00696; ETF_ALPHA 

PRINTS