CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043387
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Alanine--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
  
Gene Name
 AARS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
13DKTEFTVKNAQVRGGubiquitination[1]
74VLGEADQKGSLVAPDubiquitination[1, 2, 3, 4, 5]
90LRFDFTAKGAMSTQQubiquitination[4]
99AMSTQQIKKAEEIANubiquitination[4]
100MSTQQIKKAEEIANEubiquitination[4]
126DCPLAAAKAIQGLRAubiquitination[3, 4, 5, 6, 7, 8]
196VTEEAIAKGIRRIVAubiquitination[1, 2, 4, 5, 7, 8]
211VTGAEAQKALRKAESubiquitination[3, 4, 5, 6]
221RKAESLKKCLSVMEAubiquitination[4]
238KAQTAPNKDVQREIAubiquitination[4]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 280 AA 
Protein Sequence
XDSSEDKTEF TVKNAQVRGG YVLHIGTIYG DLKVGDQVWL FIDEPRRRPI MSNHTATHIL 60
NFALRSVLGE ADQKGSLVAP DRLRFDFTAK GAMSTQQIKK AEEIANEMIE AAKAVYTQDC 120
PLAAAKAIQG LRAVFDETYP DPVRVVSIGV PVSELLDDPS GPAGSLTSVE FCGGTHLRNS 180
SHAGAFVIVT EEAIAKGIRR IVAVTGAEAQ KALRKAESLK KCLSVMEAKV KAQTAPNKDV 240
QREIADLGEP CSRDPMDPVL GPWTFPAAPS AASRRALPKH 280 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0004813; F:alanine-tRNA ligase activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0006419; P:alanyl-tRNA aminoacylation; IEA:InterPro. 
Interpro
 IPR002318; Ala-tRNA-lgiase_IIc.
 IPR018165; Ala-tRNA-synth_IIc_core.
 IPR018163; Thr/Ala-tRNA-synth_IIc_edit.
 IPR012947; tRNA_SAD. 
Pfam
 PF07973; tRNA_SAD 
SMART
 SM00863; tRNA_SAD 
PROSITE
 PS50860; AA_TRNA_LIGASE_II_ALA 
PRINTS