UniProt Accession

 YAGE_ECOLI; P75682; Q9R2D5 

Genbank Protein ID

 U00096; AP009048 

Genbank Nucleotide ID

 AAC73371.2; BAA77934.2 

Protein Name

 Probable 2-keto-3-deoxy-galactonate aldolase YagE 

Protein Synonyms/Alias

 Probable KDGal aldolase YagE 

Gene Name

 yagE 

Gene Synonyms/Alias

 b0268; JW0261 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
167	RSNIIGIKDTIDSVA	acetylation	[1, 2]
289	SPLDEPRKAQLKTLL	acetylation	[1]
293	EPRKAQLKTLLQQLK	acetylation	[1]
300	KTLLQQLKLC*****	acetylation	[1]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the formation of 2-keto-3-deoxy-galactonate (KDGal) from pyruvate and glyceraldehyde. Overexpression leads to increased growth (over 2 hours) in the presence of the antibiotics norfloxacin, ampicillin and streptomycin. 

Sequence Annotation

 ACT_SITE 49 49 Charge relay system (Probable).
 ACT_SITE 112 112 Charge relay system (Probable).
 ACT_SITE 138 138 Proton donor (Probable).
 ACT_SITE 167 167 Schiff-base intermediate with substrate.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Lyase; Reference proteome; Schiff base. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 302 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0042802; F:identical protein binding; IDA:EcoCyc.
 GO:0016829; F:lyase activity; IEA:UniProtKB-KW. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR002220; Dihydrodipicolinate_synth-like.
 IPR020625; Dihydrodipicolinate_synth_AS.
 IPR020624; Dihydrodipicolinate_synth_CS. 

Pfam

 PF00701; DHDPS 

SMART

  

PROSITE

 PS00665; DHDPS_1
 PS00666; DHDPS_2 

PRINTS

 PR00146; DHPICSNTHASE.