CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037118
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 General transcription factor IIF subunit 1 
Protein Synonyms/Alias
  
Gene Name
 GTF2F1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
27ERDLSNKKIYQEEEMubiquitination[1, 2]
61KKYGIVLKEFRPEDQubiquitination[2, 3]
84KSGRKRNKVLNHFSIubiquitination[2, 4, 5]
98IMQQRRLKDQDQDEDubiquitination[1]
322TLRAAASKLEQGKRVacetylation[5, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 432 AA 
Protein Sequence
MAFNAADKVN FATWNQARLE RDLSNKKIYQ EEEMPESGAG SEFNRKLREE ARRKKYGIVL 60
KEFRPEDQPW LLRVNGKSGR KRNKVLNHFS IMQQRRLKDQ DQDEDEEEKE KRGRRKASEL 120
RIHDLEDDLE MSSDASDASG EEGGRVPKAK KKAPLAKGGR KKKKKKGSDD EAFEDSDDGD 180
FEGQEVDYMS DGSSSSQEEP ESKAKAPQQE EGPKGVDEQS DSSEESEEEK PPEEDKEEEE 240
EKKAPTPQEK KRRKDSSEES DSSEESDIDS EASSALFMAK KKTPPKRERK PSGGSSRGNS 300
RPGTPSAEGG STSSTLRAAA SKLEQGKRVS EMPAAKRLRL DTGPQSLSGK STPQPPSGKT 360
TPNSGDVQVT EDAVRRYLTR KPMTTKDLLK KFQTKKTGLS SEQTVNVLAQ ILKRLNPERK 420
MINDKMHFSL KE 432 
Gene Ontology
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:InterPro.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; IEA:InterPro. 
Interpro
 IPR008851; TFIIF-alpha.
 IPR011039; TFIIF_interaction.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF05793; TFIIF_alpha 
SMART
  
PROSITE
  
PRINTS