CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011441
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase PIB1 
Protein Synonyms/Alias
 Phosphatidylinositol 3-phosphate-binding protein 1 
Gene Name
 PIB1 
Gene Synonyms/Alias
 YDR313C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
4****MVIKEDCINNLubiquitination[1]
68RVHALQKKNSDVESPubiquitination[2]
172PDEEETRKHVEDCIQubiquitination[2]
199DAADDSVKESPAFQNubiquitination[2]
223NTTDNAIKECPICFEubiquitination[2]
267AQMTAAQKGNGHAFVubiquitination[2]
Reference
 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Functions as an E3 ubiquitin-protein ligase. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate. 
Sequence Annotation
 ZN_FING 17 88 FYVE-type.
 ZN_FING 225 283 RING-type; atypical.  
Keyword
 Complete proteome; Endosome; Ligase; Membrane; Metal-binding; Reference proteome; Ubl conjugation pathway; Vacuole; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 286 AA 
Protein Sequence
MVIKEDCINN LARWQADEEA HSCFQCKTNF SFLVRRHHCR CCGRIFCSSC TENFVNYNKK 60
RVHALQKKNS DVESPPYRTC NECYDNLLHL NLLVSSTNRD VRLSQTSVPP NALALSAPDS 120
NTDEDAEILE DSVDQSGTAC RSEESSQNEE DHFCPICNSD LTQFPDEEET RKHVEDCIQR 180
AENAQQHTNT SDAADDSVKE SPAFQNRMLV YKISPNTTDN AIKECPICFE NMEPGEKVGR 240
LECLCVFHYK CIKNWFHKRA QMTAAQKGNG HAFVKRNFCP FHDAVF 286 
Gene Ontology
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
 GO:0005770; C:late endosome; IDA:SGD.
 GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:SGD.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR000306; Znf_FYVE.
 IPR017455; Znf_FYVE-rel.
 IPR011011; Znf_FYVE_PHD.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF01363; FYVE
 PF13639; zf-RING_2 
SMART
 SM00064; FYVE
 SM00184; RING 
PROSITE
 PS50178; ZF_FYVE
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS