CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015582
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 PAX-interacting protein 1 
Protein Synonyms/Alias
 PAX transactivation activation domain-interacting protein 
Gene Name
 PAXIP1 
Gene Synonyms/Alias
 PAXIP1L; PTIP; CAGF28 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MSDQAPKVPEEMFRubiquitination[1]
33PQVIQLLKAGKAKEVubiquitination[1]
36IQLLKAGKAKEVSYNubiquitination[1]
630KQLLATWKRIIQAHGubiquitination[1]
749SNTVLICKEPTGLKYubiquitination[1]
847ANVQPSSKRARIEDVubiquitination[1]
938EECFRCQKFIDEQNYubiquitination[1]
974AHVSPLFKAKYFYITubiquitination[1]
1001IVECAGGKVLSKQPSubiquitination[1]
1005AGGKVLSKQPSFRKLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. Plays a role in early development. In DNA damage response is required for cell survival after ionizing radiation. In vitro shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks (DSBs). Its localization to DNA damage foci requires RNF8 and UBE2N. Recruits TP53BP1 to DNA damage foci and, at least in particular repair processes, effective DNA damage response appears to require the association with TP53BP1 phosphorylated by ATM at 'Ser-25'. Together with TP53BP1 regulates ATM association. Recruits PAGR1 to sites of DNA damage and the PAGR1:PAXIP1 complex is required for cell survival in response to DNA damage; the function is probbaly independent of MLL-containing histone methyltransferase (HMT) complexes. Promotes ubiquitination of PCNA following UV irradiation and may regulate recruitment of polymerase eta and RAD51 to chromatin after DNA damage. Proposed to be involved in transcriptional regulation by linking MLL-containing histone methyltransferase (HMT) complexes to gene promoters by interacting with promoter-bound transcription factors such as PAX2. Associates with gene promoters that are known to be regulated by KMT2D/MLL2. During immunoglobulin class switching in activated B-cells is involved in trimethylation of histone H3 at 'Lys-4' and in transcription initiation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus; this function appears to involve the recruitment of MLL- containing HMT complexes. 
Sequence Annotation
 DOMAIN 8 93 BRCT 1.
 DOMAIN 94 183 BRCT 2.
 DOMAIN 601 694 BRCT 3.
 DOMAIN 703 779 BRCT 4.
 DOMAIN 866 947 BRCT 5.
 DOMAIN 970 1059 BRCT 6.
 REGION 94 183 Interaction with PAGR1 (By similarity).
 REGION 590 1069 Interaction with TP53BP1.
 MOTIF 702 719 Nuclear localization signal (Potential).  
Keyword
 3D-structure; Alternative splicing; Complete proteome; DNA damage; DNA recombination; DNA repair; Nucleus; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1069 AA 
Protein Sequence
MSDQAPKVPE EMFREVKYYA VGDIDPQVIQ LLKAGKAKEV SYNALASHII SEDGDNPEVG 60
EAREVFDLPV VKPSWVILSV QCGTLLPVNG FSPESCQIFF GITACLSQVS SEDRSALWAL 120
VTFYGGDCQL TLNKKCTHLI VPEPKGEKYE CALKRASIKI VTPDWVLDCV SEKTKKDEAF 180
YHPRLIIYEE EEEEEEEEEE VENEEQDSQN EGSTDEKSSP ASSQEGSPSG DQQFSPKSNT 240
EKSKGELMFD DSSDSSPEKQ ERNLNWTPAE VPQLAAAKRR LPQGKEPGLI NLCANVPPVP 300
GNILPPEVRG NLMAAGQNLQ SSERSEMIAT WSPAVRTLRN ITNNADIQQM NRPSNVAHIL 360
QTLSAPTKNL EQQVNHSQQG HTNANAVLFS QVKVTPETHM LQQQQQAQQQ QQQHPVLHLQ 420
PQQIMQLQQQ QQQQISQQPY PQQPPHPFSQ QQQQQQQAHP HQFSQQQLQF PQQQLHPPQQ 480
LHRPQQQLQP FQQQHALQQQ FHQLQQHQLQ QQQLAQLQQQ HSLLQQQQQQ QIQQQQLQRM 540
HQQQQQQQMQ SQTAPHLSQT SQALQHQVPP QQPPQQQQQQ QPPPSPQQHQ LFGHDPAVEI 600
PEEGFLLGCV FAIADYPEQM SDKQLLATWK RIIQAHGGTV DPTFTSRCTH LLCESQVSSA 660
YAQAIRERKR CVTAHWLNTV LKKKKMVPPH RALHFPVAFP PGGKPCSQHI ISVTGFVDSD 720
RDDLKLMAYL AGAKYTGYLC RSNTVLICKE PTGLKYEKAK EWRIPCVNAQ WLGDILLGNF 780
EALRQIQYSR YTAFSLQDPF APTQHLVLNL LDAWRVPLKV SAELLMSIRL PPKLKQNEVA 840
NVQPSSKRAR IEDVPPPTKK LTPELTPFVL FTGFEPVQVQ QYIKKLYILG GEVAESAQKC 900
THLIASKVTR TVKFLTAISV VKHIVTPEWL EECFRCQKFI DEQNYILRDA EAEVLFSFSL 960
EESLKRAHVS PLFKAKYFYI TPGICPSLST MKAIVECAGG KVLSKQPSFR KLMEHKQNSS 1020
LSEIILISCE NDLHLCREYF ARGIDVHNAE FVLTGVLTQT LDYESYKFN 1069 
Gene Ontology
 GO:0035097; C:histone methyltransferase complex; IDA:UniProtKB.
 GO:0016363; C:nuclear matrix; IEA:UniProtKB-SubCell.
 GO:0060612; P:adipose tissue development; IEA:Compara.
 GO:0060717; P:chorion development; IEA:Compara.
 GO:0030330; P:DNA damage response, signal transduction by p53 class mediator; IMP:UniProtKB.
 GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0043542; P:endothelial cell migration; IEA:Compara.
 GO:0051568; P:histone H3-K4 methylation; IDA:UniProtKB.
 GO:0035066; P:positive regulation of histone acetylation; ISS:UniProtKB.
 GO:0000416; P:positive regulation of histone H3-K36 methylation; ISS:UniProtKB.
 GO:0051571; P:positive regulation of histone H3-K4 methylation; ISS:UniProtKB.
 GO:0045830; P:positive regulation of isotype switching; ISS:UniProtKB.
 GO:0031398; P:positive regulation of protein ubiquitination; ISS:UniProtKB.
 GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IMP:UniProtKB.
 GO:0010212; P:response to ionizing radiation; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0001570; P:vasculogenesis; IEA:Compara. 
Interpro
 IPR001357; BRCT_dom. 
Pfam
 PF00533; BRCT
 PF12738; PTCB-BRCT 
SMART
 SM00292; BRCT 
PROSITE
 PS50172; BRCT 
PRINTS