CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043658
UniProt Accession
Genbank Protein ID
 Z98046 
Genbank Nucleotide ID
  
Protein Name
 Melanoma-associated antigen D2 
Protein Synonyms/Alias
  
Gene Name
 MAGED2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
189TGGRRVSKALMASMAubiquitination[1, 2]
243KARRRAAKLQSSQEPubiquitination[3]
270GRANDLVKYLLAKDQubiquitination[1, 3]
275LVKYLLAKDQTKIPIubiquitination[2]
312RAGYSLEKVFGIQLKubiquitination[1, 2, 3]
379VIWEVLRKLGLRPGIubiquitination[1]
406TDEFVKQKYLDYARVubiquitination[2]
435RSYYETSKMKVLKFAubiquitination[2]
451KVQKKDPKEWAAQYRubiquitination[1, 2, 3, 4]
466EAMEADLKAAAEAAAubiquitination[2, 3, 4, 5]
476AEAAAEAKARAEIRAubiquitination[2, 3, 4, 6]
510ADIGPWAKARIQAGAubiquitination[2, 7]
520IQAGAEAKAKAQESGubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 588 AA 
Protein Sequence
MSDTSESGAG LTRFQAEASE KDSSSMMQTL LTVTQNVEVP ETPKASKATE VSKTPEAREA 60
PATQASSTTQ LTDTQVLAAE NKSLAADTKK QNADPQAVTM PATETKKVSH VADTKVNTKA 120
QETEAAPSQA PADEPEPESA AAQSQENQDT RPKVKAKKAR KVKHLDGEED GSSDQSQASG 180
TTGGRRVSKA LMASMARRAS RGPIAFWARR ASRTRLAAWA RRALLSLRSP KARRGKARRR 240
AAKLQSSQEP EAPPPRDVAL LQGRANDLVK YLLAKDQTKI PIKRSDMLKD IIKEYTDVYP 300
EIIERAGYSL EKVFGIQLKE IDKNDHLYIL LSTLEPTDAG ILGTTKDSPK LGLLMVLLSI 360
IFMNGNRSSE AVIWEVLRKL GLRPGIHHSL FGDVKKLITD EFVKQKYLDY ARVPNSNPPE 420
YEFFWGLRSY YETSKMKVLK FACKVQKKDP KEWAAQYREA MEADLKAAAE AAAEAKARAE 480
IRARMGIGLG SENAAGPCNW DEADIGPWAK ARIQAGAEAK AKAQESGSAS TGASTSTNNS 540
ASASASTSGG FSAGASLTAT LTFGLFAGLG GAGASTSGSS GACGFSYK 588 
Gene Ontology
  
Interpro
 IPR002190; MAGE. 
Pfam
 PF01454; MAGE 
SMART
  
PROSITE
 PS50838; MAGE 
PRINTS