CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022264
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Asparagine synthetase domain-containing protein 1 
Protein Synonyms/Alias
 HCV NS3-transactivated protein 1 
Gene Name
 ASNSD1 
Gene Synonyms/Alias
 NS3TP1; Nbla00058 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
399AAADSPNKHVSVPDRubiquitination[1]
414ITGRAGLKELQAVSPubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
 DOMAIN 2 184 Glutamine amidotransferase type-2.
 DOMAIN 285 601 Asparagine synthetase.
 ACT_SITE 2 2 For GATase activity (By similarity).  
Keyword
 Amino-acid biosynthesis; Asparagine biosynthesis; Complete proteome; Glutamine amidotransferase; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 643 AA 
Protein Sequence
MCGICCSVNF SAEHFSQDLK EDLLYNLKQR GPNSSKQLLK SDVNYQCLFS AHVLHLRGVL 60
TTQPVEDERG NVFLWNGEIF SGIKVEAEEN DTQILFNYLS SCKNESEILS LFSEVQGPWS 120
FIYYQASSHY LWFGRDFFGR RSLLWHFSNL GKSFCLSSVG TQTSGLANQW QEVPASGLFR 180
IDLKSTVISG CIILQLYPWK YISRENIIEE NVNSLSQISA DLPAFVSVVA NEAKLYLEKP 240
VVPLNMMLPQ AALETHCSNI SNVPPTREIL QVFLTDVHMK EVIQQFIDVL SVAVKKRVLC 300
LPRDENLTAN EVLKTCDRKA NVAILFSGGI DSMVIATLAD RHIPLDEPID LLNVAFIAEE 360
KTMPTTFNRE GNKQKNKCEI PSEEFSKDVA AAAADSPNKH VSVPDRITGR AGLKELQAVS 420
PSRIWNFVEI NVSMEELQKL RRTRICHLIR PLDTVLDDSI GCAVWFASRG IGWLVAQEGV 480
KSYQSNAKVV LTGIGADEQL AGYSRHRVRF QSHGLEGLNK EIMMELGRIS SRNLGRDDRV 540
IGDHGKEARF PFLDENVVSF LNSLPIWEKA NLTLPRGIGE KLLLRLAAVE LGLTASALLP 600
KRAMQFGSRI AKMEKINEKA SDKCGRLQIM SLENLSIEKE TKL 643 
Gene Ontology
 GO:0004066; F:asparagine synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
 GO:0006529; P:asparagine biosynthetic process; IEA:UniProtKB-KW.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW. 
Interpro
 IPR001962; Asn_synthase.
 IPR017932; GATase_2_dom.
 IPR000583; GATase_dom.
 IPR014729; Rossmann-like_a/b/a_fold. 
Pfam
 PF00733; Asn_synthase
 PF13537; GATase_7 
SMART
  
PROSITE
 PS51278; GATASE_TYPE_2 
PRINTS