CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000806
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 H/ACA ribonucleoprotein complex subunit 4 
Protein Synonyms/Alias
 Nucleolar protein AT band 60B; Protein minifly 
Gene Name
 Nop60B 
Gene Synonyms/Alias
 mfl; CG3333 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
16EKKKKKIKEEPLDGDacetylation[1]
395KALIAAGKLDKFGRPacetylation[1]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 
Functional Description
 Plays a central role in ribosomal RNA processing. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs. Required for maintenance of the germline stem cell lineage during spermatogenesis. 
Sequence Annotation
 DOMAIN 294 369 PUA.
 ACT_SITE 123 123 Nucleophile (By similarity).
 MOD_RES 442 442 Phosphoserine.
 MOD_RES 443 443 Phosphothreonine.
 MOD_RES 444 444 Phosphoserine.
 MOD_RES 445 445 Phosphoserine.
 MOD_RES 449 449 Phosphothreonine.
 MOD_RES 455 455 Phosphoserine.
 MOD_RES 458 458 Phosphothreonine.  
Keyword
 Alternative splicing; Complete proteome; Isomerase; Nucleus; Phosphoprotein; Reference proteome; Ribonucleoprotein; Ribosome biogenesis; RNA-binding; rRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 508 AA 
Protein Sequence
MADVEVRKEK KKKKIKEEPL DGDDIGTLQK QGNFQIKPSS KIAELDTSQW PLLLKNFDKL 60
NIRSNHYTPL AHGSSPLNRD IKEYMKTGFI NLDKPSNPSS HEVVAWIKKI LKVEKTGHSG 120
TLDPKVTGCL IVCIDRATRL VKSQQSAGKE YVAIFKLHGA VESVAKVRQG LEKLRGALFQ 180
RPPLISAVKR QLRVRTVYDS KLLDYDETRN MGVFWVSCEA GSYIRTMCVH LGLVLGVGGQ 240
MLELRRVRSG IQSERDGMVT MHDVLDAMWL YENHKDESML RRVIKPLEGL LVNHKRIIMK 300
DSSVNAVCYG AKITLPGVLR YEDGIEIDQE IVICTTKGEA ICLAIALMTT ATMASCDHGV 360
VAKIKRVIME RDTYPRKWGL GPKASAKKAL IAAGKLDKFG RPNENTPKEW LTGYVDYNAK 420
KPAAQEVSPT NGSSEPSKRK LSTSSVEETA AAAVSEETPS KDKKKKKKKH KGDEEAPEAA 480
EEEAEPVEKE KKKKKKKDKD RDRDEAQE 508 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:FlyBase.
 GO:0030529; C:ribonucleoprotein complex; IEA:UniProtKB-KW.
 GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0007281; P:germ cell development; IMP:FlyBase.
 GO:0022008; P:neurogenesis; IMP:FlyBase.
 GO:0001522; P:pseudouridine synthesis; IMP:FlyBase.
 GO:0006364; P:rRNA processing; IMP:FlyBase.
 GO:0035220; P:wing disc development; IMP:FlyBase. 
Interpro
 IPR012960; Dyskerin-like.
 IPR002501; PsdUridine_synth.
 IPR020103; PsdUridine_synth_cat_dom.
 IPR002478; PUA.
 IPR015947; PUA-like_domain.
 IPR004802; tRNA_PsdUridine_synth_B_fam. 
Pfam
 PF08068; DKCLD
 PF01472; PUA
 PF01509; TruB_N 
SMART
 SM00359; PUA 
PROSITE
 PS50890; PUA 
PRINTS