CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001311
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Wolframin 
Protein Synonyms/Alias
  
Gene Name
 WFS1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
252DDFVEITKKYAKGVIubiquitination[1]
256EITKKYAKGVIPSSLubiquitination[1, 2]
277DDDELAGKSPEDLPLubiquitination[1, 2]
679LCGPRAWKETNMARTubiquitination[1, 3]
800REEDDVTKDIVLRASubiquitination[1, 2, 4]
811LRASSEFKSVLLSLRubiquitination[1]
836LEGRLGSKWPVFELKubiquitination[1, 2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Participates in the regulation of cellular Ca(2+) homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca(2+) store. 
Sequence Annotation
 MOD_RES 32 32 Phosphoserine (By similarity).
 CARBOHYD 661 661 N-linked (GlcNAc...) (Probable).
 CARBOHYD 746 746 N-linked (GlcNAc...) (Probable).  
Keyword
 Complete proteome; Deafness; Diabetes insipidus; Diabetes mellitus; Disease mutation; Endoplasmic reticulum; Glycoprotein; Membrane; Non-syndromic deafness; Phosphoprotein; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 890 AA 
Protein Sequence
MDSNTAPLGP SCPQPPPAPQ PQARSRLNAT ASLEQERSER PRAPGPQAGP GPGVRDAAAP 60
AEPQAQHTRS RERADGTGPT KGDMEIPFEE VLERAKAGDP KAQTEVGKHY LQLAGDTDEE 120
LNSCTAVDWL VLAAKQGRRE AVKLLRRCLA DRRGITSENE REVRQLSSET DLERAVRKAA 180
LVMYWKLNPK KKKQVAVAEL LENVGQVNEH DGGAQPGPVP KSLQKQRRML ERLVSSESKN 240
YIALDDFVEI TKKYAKGVIP SSLFLQDDED DDELAGKSPE DLPLRLKVVK YPLHAIMEIK 300
EYLIDMASRA GMHWLSTIIP THHINALIFF FIVSNLTIDF FAFFIPLVIF YLSFISMVIC 360
TLKVFQDSKA WENFRTLTDL LLRFEPNLDV EQAEVNFGWN HLEPYAHFLL SVFFVIFSFP 420
IASKDCIPCS ELAVITGFFT VTSYLSLSTH AEPYTRRALA TEVTAGLLSL LPSMPLNWPY 480
LKVLGQTFIT VPVGHLVVLN VSVPCLLYVY LLYLFFRMAQ LRNFKGTYCY LVPYLVCFMW 540
CELSVVILLE STGLGLLRAS IGYFLFLFAL PILVAGLALV GVLQFARWFT SLELTKIAVT 600
VAVCSVPLLL RWWTKASFSV VGMVKSLTRS SMVKLILVWL TAIVLFCWFY VYRSEGMKVY 660
NSTLTWQQYG ALCGPRAWKE TNMARTQILC SHLEGHRVTW TGRFKYVRVT DIDNSAESAI 720
NMLPFFIGDW MRCLYGEAYP ACSPGNTSTA EEELCRLKLL AKHPCHIKKF DRYKFEITVG 780
MPFSSGADGS RSREEDDVTK DIVLRASSEF KSVLLSLRQG SLIEFSTILE GRLGSKWPVF 840
ELKAISCLNC MAQLSPTRRH VKIEHDWRST VHGAVKFAFD FFFFPFLSAA 890 
Gene Ontology
 GO:0030425; C:dendrite; ISS:BHF-UCL.
 GO:0030176; C:integral to endoplasmic reticulum membrane; IDA:BHF-UCL.
 GO:0033613; F:activating transcription factor binding; ISS:BHF-UCL.
 GO:0005215; F:transporter activity; ISS:BHF-UCL.
 GO:0031625; F:ubiquitin protein ligase binding; ISS:BHF-UCL.
 GO:0006987; P:activation of signaling protein activity involved in unfolded protein response; TAS:Reactome.
 GO:0032469; P:endoplasmic reticulum calcium ion homeostasis; IDA:BHF-UCL.
 GO:0006983; P:ER overload response; TAS:BHF-UCL.
 GO:0030433; P:ER-associated protein catabolic process; ISS:BHF-UCL.
 GO:0042593; P:glucose homeostasis; IMP:BHF-UCL.
 GO:0001822; P:kidney development; IMP:BHF-UCL.
 GO:0043524; P:negative regulation of neuron apoptotic process; IMP:BHF-UCL.
 GO:0043433; P:negative regulation of sequence-specific DNA binding transcription factor activity; ISS:BHF-UCL.
 GO:2000675; P:negative regulation of type B pancreatic cell apoptotic process; IEA:Compara.
 GO:0070845; P:polyubiquitinated misfolded protein transport; ISS:BHF-UCL.
 GO:0051928; P:positive regulation of calcium ion transport; IDA:BHF-UCL.
 GO:0045927; P:positive regulation of growth; ISS:BHF-UCL.
 GO:0031398; P:positive regulation of protein ubiquitination; ISS:BHF-UCL.
 GO:0045862; P:positive regulation of proteolysis; ISS:BHF-UCL.
 GO:0022417; P:protein maturation by protein folding; IC:BHF-UCL.
 GO:0050821; P:protein stabilization; ISS:BHF-UCL.
 GO:0003091; P:renal water homeostasis; IMP:BHF-UCL.
 GO:0007605; P:sensory perception of sound; IMP:BHF-UCL.
 GO:0007601; P:visual perception; IMP:BHF-UCL. 
Interpro
 IPR026208; Wolframin.
 IPR026209; Wolframin_fam. 
Pfam
  
SMART
  
PROSITE
  
PRINTS
 PR02060; WOLFFAMILY.
 PR02061; WOLFRAMIN.