CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032292
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ER degradation-enhancing alpha-mannosidase-like protein 3 
Protein Synonyms/Alias
 Uncharacterized protein 
Gene Name
 EDEM3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
146MAKQLGYKLLPAFNTubiquitination[1]
165PYPRINLKFGIRKPEubiquitination[1]
219ALDFLWEKRQRSSNLubiquitination[1]
392TLIENLNKYARVPCGubiquitination[1, 2]
404PCGFAAMKDVRTGSHubiquitination[1]
512PLKNVVDKSCPRGIIubiquitination[1, 3]
551EHLEILKKMGVSLIHubiquitination[1]
662NPEAVMGKIALIQRGubiquitination[1]
676GQCMFAEKARNIQNAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 905 AA 
Protein Sequence
MSREEKQKLG NQVLEMFDHA YGNYMEHAYP ADELMPLTCR GRVRGQEPSR GDVDDALGKF 60
SLTLIDSLDT LVVLNKTKEF EDAVRKVLRD VNLDNDVVVS VFETNIRVLG GLLGGHSLAI 120
MLKEKGEYMQ WYNDELLQMA KQLGYKLLPA FNTTSGLPYP RINLKFGIRK PEARTGTETD 180
TCTACAGTLI LEFAALSRFT GATIFEEYAR KALDFLWEKR QRSSNLVGVT INIHTGDWVR 240
KDSGVGAGID SYYEYLLKAY VLLGDDSFLE RFNTHYDAIM RYISQPPLLL DVHIHKPMLN 300
ARTWMDALLA FFPGLQVLKG DIRPAIETHE MLYQVIKKHN FLPEAFTTDF RVHWAQHPLR 360
PEFAESTYFL YKATGDPYYL EVGKTLIENL NKYARVPCGF AAMKDVRTGS HEDRMDSFFL 420
AEMFKYLYLL FADKEDIIFD IEDYIFTTEA HLLPLWLSTT NQSISKKNTT SEYTELDDSN 480
FDWTCPNTQI LFPNDPLYAQ SIREPLKNVV DKSCPRGIIR VEESFRSGAK PPLRARDFMA 540
TNPEHLEILK KMGVSLIHLK DGRVQLVQHA IQAASSIDAE DGLRFMQEMI ELSSQQQKEQ 600
QLPPRAVQIV SHPFFGRVVL TAGPAQFGLD LSKHKETRGF VASSKPSNGC SELTNPEAVM 660
GKIALIQRGQ CMFAEKARNI QNAGAIGGIV IDDNEGSSSD TAPLFQMAGD GKDTDDIKIP 720
MLFLFSKEGS IILDAIREYE EVEVLLSDKA KDRAAILKGK MIPSYIINSN PEMENEEQPS 780
SENDSQNQSG EQISSSSQEV DLVDQESSEE NSLNSHPESL SLADMDNAAS ISPSEQTSNP 840
TENHETTNLN GECTDLDNQL QEQSETEEDS NPNVSWGKKV QPIDSILADW NEDIEAFEMM 900
EKDEL 905 
Gene Ontology
 GO:0016020; C:membrane; IEA:InterPro.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0004571; F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity; IEA:InterPro. 
Interpro
 IPR001382; Glyco_hydro_47.
 IPR003137; Protease-assoc_domain. 
Pfam
 PF01532; Glyco_hydro_47
 PF02225; PA 
SMART
  
PROSITE
  
PRINTS
 PR00747; GLYHDRLASE47.