CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021164
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribosome biogenesis protein WDR12 
Protein Synonyms/Alias
 WD repeat-containing protein 12 
Gene Name
 WDR12 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
121ILTGSYDKTSRIWSLubiquitination[1, 2]
203AVDGSGTKFCSGSWDubiquitination[1, 2, 3]
211FCSGSWDKMLKIWSTubiquitination[1]
239NRPRKKQKTEQLGLTubiquitination[2]
291DVESGSLKSTLTGNKubiquitination[2]
310ISYSPLCKRLASGSTubiquitination[1]
388DLAAHEDKVLSVDWTubiquitination[4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. 
Sequence Annotation
 REPEAT 99 137 WD 1.
 REPEAT 138 180 WD 2.
 REPEAT 187 226 WD 3.
 REPEAT 255 293 WD 4.
 REPEAT 295 334 WD 5.
 REPEAT 340 380 WD 6.
 REPEAT 384 422 WD 7.
 REGION 98 423 Sufficient for nucleolar localization.
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 Acetylation; Complete proteome; Nucleus; Polymorphism; Reference proteome; Repeat; Ribosome biogenesis; rRNA processing; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 423 AA 
Protein Sequence
MAQLQTRFYT DNKKYAVDDV PFSIPAASEI ADLSNIINKL LKDKNEFHKH VEFDFLIKGQ 60
FLRMPLDKHM EMENISSEEV VEIEYVEKYT APQPEQCMFH DDWISSIKGA EEWILTGSYD 120
KTSRIWSLEG KSIMTIVGHT DVVKDVAWVK KDSLSCLLLS ASMDQTILLW EWNVERNKVK 180
ALHCCRGHAG SVDSIAVDGS GTKFCSGSWD KMLKIWSTVP TDEEDEMEES TNRPRKKQKT 240
EQLGLTRTPI VTLSGHMEAV SSVLWSDAEE ICSASWDHTI RVWDVESGSL KSTLTGNKVF 300
NCISYSPLCK RLASGSTDRH IRLWDPRTKD GSLVSLSLTS HTGWVTSVKW SPTHEQQLIS 360
GSLDNIVKLW DTRSCKAPLY DLAAHEDKVL SVDWTDTGLL LSGGADNKLY SYRYSPTTSH 420
VGA 423 
Gene Ontology
 GO:0005654; C:nucleoplasm; IDA:UniProtKB.
 GO:0070545; C:PeBoW complex; IDA:UniProtKB.
 GO:0030687; C:preribosome, large subunit precursor; IDA:UniProtKB.
 GO:0008283; P:cell proliferation; IMP:UniProtKB.
 GO:0000466; P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:UniProtKB.
 GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:UniProtKB.
 GO:0007219; P:Notch signaling pathway; IEA:Compara. 
Interpro
 IPR020472; G-protein_beta_WD-40_rep.
 IPR012972; NLE.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF08154; NLE
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.