UniProt Accession

 MCR1_YEAST; P36060; A2TBM9; D6VX47 

Genbank Protein ID

 X81474; Z26877; Z28150; EF123132; BK006944 

Genbank Nucleotide ID

 CAA57227.1; CAA81503.1; CAA81991.1; ABM97476.1; DAA09013.1 

Protein Name

 NADH-cytochrome b5 reductase 2 

Protein Synonyms/Alias

 Mitochondrial cytochrome b reductase; p34/p32; NADH-cytochrome b5 reductase p34 form; NADH-cytochrome b5 reductase p32 form 

Gene Name

 MCR1 

Gene Synonyms/Alias

 YKL150W; YKL605 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
138	TSHLFGLKPNDTVSF	acetylation	[1]
146	PNDTVSFKGPIMKWK	acetylation	[1]
153	KGPIMKWKWQPNQFK	acetylation	[1]
296	LNNLGYSKDQVFKF*	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 The outer membrane form may mediate the reduction of outer membrane cytochrome b5, and the soluble inter-membrane space form may transfer electrons from external NADH to cytochrome c, thereby mediating an antimycin-insensitive, energy-coupled oxidation of external NADH by yeast mitochondria. Involved in the reduction of D-erythroascorbyl free radicals. 

Sequence Annotation

 DOMAIN 51 155 FAD-binding FR-type.
 NP_BIND 158 193 FAD (By similarity).
 MOD_RES 278 278 Phosphoserine.  

Keyword

 Complete proteome; Direct protein sequencing; FAD; Flavoprotein; Membrane; Mitochondrion; Mitochondrion outer membrane; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 302 AA 

Protein Sequence

Gene Ontology

 GO:0031307; C:integral to mitochondrial outer membrane; IDA:SGD.
 GO:0005758; C:mitochondrial intermembrane space; IDA:SGD.
 GO:0004128; F:cytochrome-b5 reductase activity, acting on NAD(P)H; IDA:SGD.
 GO:0034599; P:cellular response to oxidative stress; IMP:SGD.
 GO:0006696; P:ergosterol biosynthetic process; IDA:SGD. 

Interpro

 IPR017927; Fd_Rdtase_FAD-bd.
 IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
 IPR001834; NADH-Cyt_B5_reductase.
 IPR008333; OxRdtase_FAD-bd_dom.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR017938; Riboflavin_synthase-like_b-brl. 

Pfam

 PF00970; FAD_binding_6
 PF00175; NAD_binding_1 

SMART

  

PROSITE

 PS51384; FAD_FR 

PRINTS

 PR00406; CYTB5RDTASE.
 PR00371; FPNCR.